摘要
目的通过观察犀角地黄汤对庆大霉素引起视网膜血管损伤的影响来评价其疗效,为中药治疗视网膜血管性疾病尤其是视网膜血管炎提供新的研究思路和方法。方法用犀角地黄汤、地塞米松和生理盐水作为处理因素,玻璃体腔注射500ug庆大霉素的青紫蓝兔作为处理对象,通过眼底彩照、荧光素眼底血管造影、视网膜血管消化铺片、光镜及电镜标本制作、免疫组织化学检测细胞间黏附分子-1含量来观察不同处理因素作用后7天及14天各组兔视网膜及血管等变化。结果中药中剂量对500ug庆大霉素致视网膜及血管的损伤有明显的治疗作用,能有效减轻视网膜出血、缓解炎症反应以及降低细胞间黏附分子-1的表达,且随时间延长疗效稳定。中药大、小剂量仅能改善视网膜出血情况。地塞米松早期可明显减少出血及抑制炎症细胞数量,但长时间应用后加重了炎症反应。结论犀角地黄汤作为凉血清热,化斑散瘀的经典方,能有效控制庆大霉素致家兔视网膜出血及炎症反应,且没有明显副作用,这为临床相关疾病,尤其是视网膜血管炎的中医药治疗提供了实验研究基础。
OBJECTIVE To study the protection of Xijiao Dihuang decoction on gentamincin affected Rabbit＇s retinal blood vessels, then identify the therapeutic effects of this Chinese medicinal herbs on this kind of and other similar diseases, such as retinal vasculitis. METHODS Five hundred micrograms gentamincin was injected into rabbits＇ vitreous body, and then the rabbits were treated with Xijiao Dihuang decoction, dexamethasone or saline. All the eyes were measured with ocular fundus photography, fluorescein angiography, retinal vascular digestion, histopathologic finding, electron microscopy, histochemieal changes after 7 and 14 days of treatment. RESULTS The moderate dosage of Xijiao Dihuang decoction had significant effect on retina and vessels affected by gentamincin, which could lighten the hemorrhage of retina, reduce the inflammatory reaction, depress the expression of intercellular adhesion molecular-1 （ICAM-1）, the effective treatment was very stable even after long time. The small and big dosage of Xijiao Dihuang decoction could only lighten the hemorrhage of retina. Dexamethasone had obvious effect at the first 7 days. But after longer treatment, it aggravated the inflammatory reactions. CONCLUSIONS As a classical formula herb, Xijiao Dihuang decoction could decrease the hemorrhage and inflammatory reaction of the rabbits＇ retina and blood vessels induced by gentamicin, having no obvious side-effect. It would be of great value in clinical practice about the similar diseases especially on retinal vasculitis.
出处
《中国中医眼科杂志》
2008年第5期253-256,F0002,共5页
China Journal of Chinese Ophthalmology
关键词
庚大霉素
视网膜
血管炎
犀角地黄汤
gentamicin
retina
vasculitis
Xijiao Dihuang decoction
