摘要
根据BglII和BamHI同尾酶的特点,通过对pPIC9K/CB进行改造,获得了不含BamHI位点的CecropinB表达框,并在此基础上构建了含三拷贝Cecropin B表达框的毕赤酵母表达载体,为Cecropin B的进一步开发与应用打下了基础。
The new expression box of Cecropin B without the restriction to the site of BamH I was obtained by the transformation of pPIC9K/CB, which was based on the characteristics of isocaudamers of BglII and BamH I. The expression vector of Pichia pastoris containing three copies of Cecropin B was therefore constructed, which laid the foundations for farther development and applications of Cecropin B.
出处
《山东科学》
CAS
2008年第5期21-24,共4页
Shandong Science
基金
国家高技术研究发展计划(863计划)现代农业技术领域重大项目(2006AA10A211)
关键词
天蚕素B
毕赤酵母
多拷贝
载体构建
Cecmpin B
Pichia pastoris
repeats copy
construction of expression vector
