摘要
采用rep-PCR指纹技术，对73个重庆稻瘟病菌菌株进行了DNA指纹扩增。结果表明，菌株间显示了DNA指纹的多态性，供试菌株分别扩增出2～17条DNA带。经UPGMA聚类分析，在0．80遗传相似水平下，供试菌株分为12个遗传谱系，其中谱系L7，L9，L12为优势谱系。重庆稻瘟病菌的群体结构呈现多样性和复杂性，菌株的遗传谱系与原寄主品种和地理分布之间均表现出较明显的相关性。
DNA fingerprints of 73 Magnaporthe grisea isolates collected from the rice in Chongqing were tested with the rep-PCR technique. Polymorphism of DNA fingerprints existed among the isolates. The tested isolates showed 2-17 DNA bands after amplification. UPGMA analysis clustered 73 isolates into 12 genetic lineages at 0.8 genetic similarity. Of these lineages, LT, L9 and L12 were dominate lineages. The results indicated that genetic structure of M. grisea population in Chongqing was multiplicity and complexity, and the results also revealed that genetic lineage isolates were directly related to variety and geography distribution.
出处
《河南农业科学》
CSCD
北大核心
2008年第11期76-79,共4页
Journal of Henan Agricultural Sciences
基金
重庆市自然科学基金项目(CSTC,2006BB1318)
