摘要
以1对同核异质玉米C103自交系为试材,采用离体叶片法检测适宜浓度的玉米小斑病菌C小种(HMC)毒素培养滤液来诱导玉米的抗病性。结果表明:对照的玉米病斑面积是处理组的7.9～12.2倍,差异极显著,并以1∶60预处理效果最佳;经0～72 h的动态检测,与对照相比,诱导玉米的过氧化物酶活性平均提高了57.8%,苯丙氨酸解氨酶活性平均提高了52.9%,有害物质丙二醛的含量平均降低了38.2%;低浓度HMC毒素培养滤液能够作为激发子诱导专化寄主玉米获得抗病性。
In the experiment induced disease-resistance of maize by culture filtrate of HMC toxin was investigated. A pair of homokaryon maize C103 inbred lines were used as the test material. The pathogenicity was measured by the detached leaf method in order to find moderately diluted concentration for inducing disease-resistance. The results showed that lesion area decreased by 7. 9- 12. 2 times. It was significantly different by ANOVA. Among the treatments, the effect of 1 ： 60 is better than 1 ： 50;POD and PAL activities increased by 57.8% and 52. 9 % respectively. However, MDA content decreased from by 38. 2 % compared with CK during 0-72 h inspection. In short,moderately diluted（1 ： 60） culture filtrate of HMC toxin could function as an elicitor to induce host disease-resistance in maize.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2008年第5期590-596,共7页
Journal of Huazhong Agricultural University
基金
supported by International Project of Science and Technology between South Africa and China's Mainland (2006DFB02480)
key project of Hebei Natural Science Foundation of China (C2006000744)
the project of Scientific and Technological Research and Development Hebei Province,China(07297162D)~~
关键词
玉米
HMC毒素
诱导抗性
maize
HMC toxin
induced resistance
maize
