摘要
为了建立一接近人类肾小球硬化病理特征、制作周期短、稳定性重复性良好的动物模型,以供新药研究之用,对健康雄性SD大鼠行左侧肾切除合并阿霉素双次注射尾静脉的方法建立肾小球硬化动物模型,正常组则行假手术及予等量生理盐水尾静脉注射。于第0,2,4,6,8周留取24h尿液测定尿蛋白含量;第8周末取血检测血生化指标,单侧肾切除合并阿霉素双次尾静脉注射的肾小球硬化模型大鼠的24h蛋白尿、血脂都较正常组明显升高。该模型病理特征与人类肾小球硬化相似,其建立为治疗肾小球硬化的新药开发奠定了实验基础。
An animal model similar to human＇s patho-glomerular sclerosis（GS） characteristic for reference of new drug research was established. Nephrectomizeing the left kidney of the healthy male SD rat and vena caudalis injected Adriamyein twice in order to establish a glomerular sclerosis（GS） animal model. The normal group carried out sham operation and vena caudalis injected partes aequales normal sodium. The protein level of 24hours＇ urine in 0 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks was assayed. In addition, the blood in 8 weeks was asayed. 24hours＇ albuminuria and blood-fat of the nephrectomized group were heightened obviously compared to the normal group. This patho characteristic of the animal model is similar to human＇s patho-glomerular sclerosis（GS）. The establishion provides an experimental basis for developing a new drug to treat glomerular sclerosis（GS）.
出处
《药物生物技术》
CAS
CSCD
2008年第5期398-400,共3页
Pharmaceutical Biotechnology
关键词
肾小球硬化
动物模型
大鼠
Glomerular sclerosis （GS）, Animal model, SD rat
