摘要
目的研究抗内皮细胞抗体（AECA）在系统性血管炎（SV）中的阳性率、抗原成分及临床意义。方法免疫印迹法检测系统性血管炎、系统性红斑狼疮（SLE）、类风湿关节炎（RA）、干燥综合征（pSS）及正常人血清中AECA阳性率，分析各组AECA与临床表现的相关性。结果（1）SV、SLE及SS中AECA阳性率明显高于RA及正常人组（P〈0．01）。（2）AECA为一异质性抗体，其抗原分子质量在16～110ku。SV和SLE中均有与分子质量47ku抗原结合的AECA。（3）AECA阳性的TA其血沉高于阴性组（P〈0．05）；WG、MPA、CSS中，AECA阳性者伯明翰血管炎活动性评分（BVAS）高于阴性组（P〈0．05）；BD中，47ku—AECA阳性组神经系统受损和CRP升高者多于阴性组（P〈0．05）。结论AECA与疾病活动性相关，在不同SV及SLE中可能存在共同靶抗原，且与疾病临床表现相关。
Objective To investigate the prevalence, their autoantigen and the clinical significants of antiendothelial cell antibodies （AECAs） in systemic vasculitis. Methods Western blotting was performed to detect specific AECA in serum of systemic vasculitis, SLE, RA, SS and healthy donors. Then to analyze the relationships of AECA with the disease manifestation. Results （ 1 ） The prevalence of AECA was 77.7% in systemic vasculitis, 87. 5% in SLE, 66. 7% in SS, 7. 14% in RA and 10% in normal group respectively. （2）AECA reacted with a heterogeneous series of endothelial proteins which ranged in molecular size from 16 to 120 ku Furthermore, AECA against a 47 ku endothelial cell antigen were more frequently found in a variety （ 3 ） Compared with those in AECA-negative patients, the mean levels of ESR in of systemic vasculitis and SLE. AECA-positive patients with TA and the mean levels of BVAS in patients with WG, MPA and CSS were both significantly higher in AECA-positive patients. Patients with BD who have AECA against 47 ku endothelial cell proteins were more frequently found to have neuropathy than those 47 ku-AECA-negative patients, and the prevalence of inhanced CRP are also more frequent. Conclusion AECA showed to be correlated with the disease manifestation, and the same molecular size antigen could be found in a variety of systemic vasculitis and SLE.
出处
《基础医学与临床》
CSCD
北大核心
2008年第10期1040-1043,共4页
Basic and Clinical Medicine
基金
国家自然科学基金(30400411)
关键词
内皮细胞
抗内皮细胞抗体
免疫印迹
endothelial cell
antiendothelial cell antibody
western blotting
