摘要
目的研究尾型同源盒转录因子CDX1、CDX2在不同亚型胃黏膜肠化生及胃癌组织中的表达，探讨二者在胃黏膜肠化生进展及胃癌发生中的作用。方法分别选取慢性浅表性胃炎40例，单纯慢性萎缩性胃炎（CAG）40例，CAG伴肠化生46例，胃癌40例，对应癌旁组织伴肠化生32例。分别用免疫组织化学和原位杂交方法观察CDX2蛋白及CDX1、CDX2 mRNA在上述胃黏膜病变中的表达情况。结果癌旁组织伴肠化生中Ⅲ型肠化生的比例显著高于CAG伴肠化生（分别为56．25％和21．74％，P〈0．01）；CDX1、CDX2 mRNA及CDX2蛋白在慢性浅表性胃炎及单纯CAG中均无表达，而在CAG伴肠化生、癌旁组织伴肠化生和胃癌中，CDX2蛋白及其mRNA表达阳性率分别为69．57％、53．12％、42．50％和63．04％、46．88％、35．00％，CDX1 mRNA表达阳性率分别为67．39％、50．00％、40．00％，胃癌中的表达阳性率均显著低于CAG伴肠化生（P〈0．01），而与癌旁组织伴肠化生比较差异无统计学意义（P〉0．05）；CDX2蛋白在肠型胃癌中的表达阳性率（54．55％）显著高于弥漫型胃癌（27．78％）（P〈0．05）。Ⅲ型肠化生中CDX2蛋白表达阳性率及表达强度则显著低于Ⅰ型（P〈0．05）。结论CDX1、CDX2在胃黏膜肠化生进展及胃癌发生中有重要作用，可能是新的胃癌相关基因。Ⅲ型肠化生与肠型胃癌有密切关系。
Objective To study the expressions of caudal type homeodomain transcription factors CDX1 and CDX2 in different subtype of intestinal metaplasia （IM） and gastric carcinoma,and investigate their roles in the development and progression of IM and gastric carcinogenesis, and determine the relationship between IM and gastric carcinoma. Methods Forty cases of chronic superficial gastritis, 40 cases of chronic atrophic gastritis （CAG）, 46 cases of CAG with IM, 40 cases of gastric carcinoma, and 32 cases of IM foci in para-cancerous tissues were selected respectively to construct tissue microarrays. Immunohistochemistry and in situ hybridization were used to assess the expressions of CDX2 protein and CDX1, CDX2 mRNA in different gastric lesions respectively. Results The proportion of type Ⅲ IM in IM foci in para-cancerous tissues was significantly higher than that in CAG （56.25% vs 21.74% ,P 〈 0.01 ）. There was no expression of CDX1, CDX2 mRNA and CDX2 protein in chronic superficial gastritis and CAG without IM. The expression of CDX2 protein and CDX2 mRNA in CAG with IM, para-ancerous tissues and gastric carcinoma was 69.57%, 53.12%, 42.50% （CDX2 protein） and 63.04%,46.88%,35.00% （CDX2 mR-NA）, respectively. The expressions of CDXl mRNA in above three gastric lesions were 67.39%, 50.00%, 40.00%, respectively. The expressions of CDX2 protein and CDX2, CDX1 mRNA in gastric carcinoma were significantly lower than those in CAG with IM （P 〈 0.01 ）. But there was no significant difference between gastric carcinoma and IM foci in para-cancerous tissues （P 〉 0.05 ）. Furthermore,the expressions of CDX2 protein were significantly associated with histological type of gastric carcinoma, which in intestinal-type was significantly higher than those in diffuse-type（ 54.55% vs 27.78%, P 〈 0.05 ）. The expression of CDX2 protein in type Ⅲ IM was also significantly lower than that in type Ⅰ IM（46.43% vs 79.31%, P 〈 0.05 ）. Conclusions CDX1 and CDX2 may play important roles in the developm
出处
《中国医师进修杂志（内科版）》
2008年第9期3-7,共5页
Chinese Journal of Postgraduates of Medicine
基金
卫生部临床学科重点项目（20012130）
国家自然科学基金（30572383）
关键词
肠黏膜
化生
胃肿瘤
CDX1
CDX2
Intestinal mucosa
Metaplasia
Stomach neoplasms
CDX1
CDX2
