摘要
以高纤维素素含量的土壤为筛选源,利用定向筛选技术,经过多代淘汰,最终筛选出一组木质纤维素分解混合菌群P-C。混合菌群的生长曲线没有明显的分界线,在培养4至5天时,出现了短暂的平稳期,在培养第7天时出现峰值,此时混合菌群的纤维素酶活最高。以天然纤维素为产酶碳源时,混合菌群纤维素酶活明显高于以人工纤维素为产酶碳源时的酶活,以秸秆为碳源时的纤维素酶活是以滤纸为碳源时的1.5倍。混合菌群纤维素酶的最适pH是6,最适酶促反应温度是45℃,但在35℃-40℃时也具有较高酶活。pH=6,糖化48 h时,发酵液糖浓度最高。
A microbial community with high cellulose -degradation ability was selected from soil with high cellulose content by the techniques of restricted cultivation. There was no obvious marker between different phase on the growth curve and a very short stable phase appeared between 4 and 5 days cultivation. The peak was visualized at the seventh day after inoculation when the highest cellulase activity was investigated. The cellulose degrading capacity of the community was much higher with corn stover as carbon source than with filter paper and the former cellulase activity was 1.5 times higher than the latter. The optimum pH for microbial community was 6. 0. The optimum temperature for degrading cellulose was 45 ℃, while the cellulase activity also was at high level when the temperature was keep at 35℃ -45℃. The highest glucose concentration was gained at pH = 6 after 48h saccharification.
出处
《环境科学与管理》
CAS
2008年第9期116-119,共4页
Environmental Science and Management
关键词
纤维素降解菌
混合菌群
纤维素酶活
糖化
cellulose degradation
microbial community
enzyme activity
saccharification
