摘要
以能源植物斑茅(Erianthus arundinaceus)为材料,对其纤维成分、产酶条件和糖化条件进行了分析。结果表明:斑茅含有纤维素43.77%、半纤维素28.57%、木质素11.01%;对斑茅进行预处理后,纤维中纤维素的含量提高至72.54%,半纤维素含量降低至13.02%,木质素的含量降低至3.34%。利用预处理的斑茅纤维进行固态发酵产酶,其最佳的产酶条件为:茅麸比1∶3,固液比1∶2,氮源(脲)0.75%,初始pH 5.0,32℃发酵培养72 h;相对于利用未经预处理的斑茅纤维为原料,其FPA酶活提高210.9%,达到20.74 U/g,CMC酶活提高239.1%,达到218.26 U/g。以纤维素酶对斑茅纤维进行糖化,糖化率达到40.60%。
Using energy plant Erianthus arundinaceus as the material, we analysed the fiber composition, enzyme production conditions and saccharification. The results showed that Erianthus arundinaceus consisted of 43.77% cellulose, 28.57% hemicellulose and 11.01% lignin. After pretreatment, the cellulose content increased to 72.54%, while the hemicellulose and lignin content decreased to 13.02% and 3.34%, respectively. The optimal condition for solid-state fermentation to produce cellulase was： ratio of pretreated cellulose fibre to wheat bran 1 ： 3, ratio of solid to liquid 1：2, nitrogen source（urea）0.75%, initial pH 5.0, temperature 32℃ and fermentation time 72 h. When using the pretreated fibre as the carbon source, the FPA activity and CMC activity increased 210.9% and 239.1%, respectively. The pretreated fibre was used for saccharification under the optimal condition with a.yield of 40.60%.
出处
《热带作物学报》
CSCD
2011年第12期2346-2351,共6页
Chinese Journal of Tropical Crops
基金
国际科技合作重点项目(No.2009DFA60930)
国家自然科学基金(No.31170067)资助
关键词
能源植物
斑茅
纤维素酶
固态发酵
糖化
Energy plant
Erianthus arundinaeeus
Cellulase
Solid-state fermentation
Aecharification
