摘要
目的:建立中药枇杷叶的高效毛细管电泳(HPCE)指纹图谱。方法:采用正丁醇超声提取法制备品种、批次不同的枇杷叶供试液,以熊果酸对照品为内参考物,运用高效毛细管区带电泳法测定指纹图谱,并用相似度评价软件分析其相似度。结果:所测定的13个品种枇杷叶样品,其指纹图谱相似度0.9以上有11个,0.9～0.8之间1个,0.8～0.7之间1个,指纹图谱整体面貌基本一致的。结论:所建立的枇杷叶高效毛细管电泳指纹图谱稳定、可靠,可作为枇杷叶的特征指纹图谱,为枇杷叶的定性鉴别及药材内在质量评价提供了新的参考依据。
Objective：To establish the fingerprint spectrum of loquat leaf by high performance capillary electrophoresis （HPCE）. Methods：Loquat leaf testing solution of different species and batches was got by n-butanol ultrasound with ursolic acid as control. Then the fingerprint was detected by capillary zone electrophoresis（CZE）and its similarity was analyzed by Similar Evaluated Software. Results：The similarity of the 11 fingerprint out of the 13 loquat leaf samples were above 0.9 ,one was within 0.9-0.8 ,one was within 0.8-0.7. The fingerprint was generally constant. Conclusion ：Stable and reliable, HPCE fingerprint spectrum could be regarded as fingerprint spectrum and further offer a new identificaiton and evaluation for the qualitation and quality for loquat leaf.
出处
《世界中西医结合杂志》
2008年第1期23-25,共3页
World Journal of Integrated Traditional and Western Medicine
基金
福建省重大科技攻关项目(No.2002y010)
