摘要
目的研究窄谱中波紫外线(NB-UVB)对寻常型斑块状银屑病患者血清肿瘤坏死因子-α(TNF-α)及白介素-8(IL-8)水平的影响,探讨治疗机制。方法应用窄德国全身谱中波紫外线光疗机(舱),治疗35例寻常性斑块状银屑病患者,每周照射3次,连续治疗9周为1疗程。应用双抗体夹心ELISA法,分别于治疗前、治疗后对35例寻常性斑块状银屑患者血清TNF-α及IL-8水平进行检测。结果治疗后总有效率为85.7%;治疗前患者血清TNF-α(1.07±0.15 ng/ml)及IL-8(412.56±77.37 pg/ml)水平较健康志愿者TNF-α(0.31±0.07 ng/ml),IL-8(76.03±20.75 pg/ml)水平高,差异显著(P<0.05);治疗后患者血清TNF-α(0.42±0.08 ng/ml)及IL-8(86.87±24.94 pg/ml)水平仍高于较健康志愿者,但差异不显著(P>0.05);治疗前、后比较:NB-UVB照射后血清TNF-α及IL-8水平显著下降(P<0.05)结论NB-UVB治疗寻常斑块状银屑病疗效高,不良反应少;NB-UVB对寻常银屑病(斑块状)患者血清TNF-α及IL-8水平有抑制作用。
Objective Experimental Study on the Effect of TNF-α and IL-8 in Serum of Psoriasis Vulgaris plaque With NB - UVB,To exploretherapeutic mechanism.Methods To treat 35 patients of psoriasis vulgaris plaque by NB-UBV （UV700 1） .Exposure three times in a week and continue to exposure nine weeks. Apply to ELISA. Testing serumal concentration of TNF-α and IL-8 in the pretherapy and post-treatment.Results All effective rate was up to 85.7 % in post-treatment. The mean levels of TNF-α（ 1.07±0.15 ng/ml） and IL-8（412.56±77.37 pg/ml） were significantly higher in patients in prethempythan In healthy volunteer（ P 〈 0. 05）, compired with healthy volunteer in post-treatment, patient group was high. but distinction was not obvious（ P 〉 0.05 ）. TNF-α and IL-8 were significant degression in post-treatment by the NB-UVB（ P 〈 0.05）. Conclusion The studies indicate that NB-UVB is more curative effect in the treatment of psoriasis vulgaris plaque, moreover, It was less adverse effect and recurrence rate. The levels of TNF-α and IL-8 had been inhibited in the serum by NB-UVB therapy.
出处
《中国实验诊断学》
2008年第10期1279-1280,共2页
Chinese Journal of Laboratory Diagnosis
