摘要
目的:建立离子交换色谱法检测食品添加剂富马酸中杂质马来酸。方法:样品用流动相溶解定容后,采用LC-SCX离子交换色谱柱(25cm×4.6mm,5μm)分离,以0.005mol/L硫酸水溶液-乙腈(60:40)为流动相,流速0.3mL/min,检测波长208nm。结果:样品中富马酸与马来酸分离度达到3.1,在0.1～5.0mg/L范围内,马来酸峰面积与浓度呈良好的线性关系(r=0.9999),最低检出限达到0.05g/kg,重现性良好。结论:该法简便、快速、灵敏、准确,可用于食品添加剂富马酸中马来酸的检测。
Objective： To establish a method for the determination of maleic acid in fumaric acid by ion exchange chromatography（IEC）. Methods： Samples were dissoluted by mobile phase directly. Chromatographic analysis was performed on a LC-SCX （25cm×4.6mm, 5 μm） column eluted with 0.005 mol/L sulfuric acidacetonitrile （60：40） at the flow rate of 0.3 mL/min, and detection at 208 nm, Results： The separation degree between fumaric acid and maleic acid was 3.1. The calibration curve was linear at the range of 0.1-5.0 mg/L with good precision and accuracy. The detection limit was 0.05 g/kg. Conslusion： The method was simple, rapid, sensitive and accurate. It＇s suitable for determination of maleic acid in fumaric acid as food additives.
出处
《食品科技》
CAS
北大核心
2008年第10期226-228,共3页
Food Science and Technology
基金
国家标准制标项目(20071780-T-424)
关键词
离子交换色谱
富马酸
马来酸
ion exchange chromatography
fumaric acid
maleic acid
