摘要
目的通过研究雌激素对慢性间歇性低氧（chronic intermittent hypoxia，CIH）大鼠颏舌肌线粒体细胞色素c氧化酶（cytochrome Coxidase，COX）活性及其亚基（COXI、COXIV）表达的影响，探讨阻塞性睡眠呼吸暂停低通气综合征（OSAHS）发病中雌激素所起的作用。方法3个月龄健康雄性sD大鼠48只，分为正常对照组（NC组）、慢性间歇性低氧组（CIH组）及慢性间歇性低氧＋雌激素干预组（E＋CIH组）。后两组建立CIH模型，同时E＋CIH组给予苯甲酸雌二醇0．2ms／kg。NC组、CIH组给予无菌橄榄油0．2ml／次，2次／周，肌肉注射。密度梯度离心法分离大鼠颏舌肌线粒体，极谱法测量COX活性；Western blotting分析COXI和COXIV蛋白表达；实时聚合酶链反应（realtime polymerase chain action）法检测COXI和COXIV的基因表达。结果CIH组大鼠颏舌肌线粒体COX活性为（0．143±0．029）ukat／mg，与NC组[（0．273±0．058）ukat／mg]相比显著降低（P〈0．01），E＋CIH组COX活性[（0．203±0．073）ukat/mg]较CIH组有所回升（P〈0．05），但仍显著低于NC组（P〈0．05）。CIH组与E＋CIH组COX Ⅰ蛋白表达分别为（10．789±8．144）和（25．593±11．108），与NC组（47．325±7．502）相比均显著降低（P〈0．01），且E＋CIH组COXI蛋白表达显著高于CIH组（P〈0．05）。3组COX Ⅳ蛋白表达的差异无统计学意义（P〉0．05）；3组COX Ⅰ、COX Ⅳ mRNA表达量的差异无统计学意义（P〉0．05）。结论CIH可抑制大鼠颏舌肌线粒体COX蛋白表达及活性，而雌激素干预可部分恢复COX蛋白表达及活性。
Objective To investigate the effects of estrogen on the activity and subunits expression of cytochrome c oxidase （COX） in mitochondria from genioglossus of chronic intermittent hypoxia （CIH） rat. Methods Forty-eight male Sprague-Dawley （SD） rats were randomly divided into three groups： the normal control group （NC）, the chronic intermittent hypoxia group （CIH） and estrogen plus hypoxia group （E ＋ CIH）. Rats in the latter two groups were used to build CIH modets（8 h/d,5 w）. In the mean time, rats in NC group and CIH group were injected with sterile olive oil （0. 2 ml each time, twice a week）, and rats in E ＋ CIH group were injected with estrogen （0. 2 mg/kg, twice a week）, respectively. At the end of the treatment, the genioglossus of the rat was removed and the mitochondria were isolated by centrifugation program. COX activity was measured with a spectrophotometer. The protein content of COX subunit Ⅰ and Ⅳ was detected by Western blotting analysis, and mRNA expression levels of COX Ⅰ and COX Ⅳ were determined by real-time polymerase chain action. Results COX activity in CIH group [ （0. 143 ± 0. 029）ukat/mg ] was lower significantly than that in NC group [ （ 0. 273 ± 0. 058 ） ukat/mg, P 〈 0. 01 ] ; In E ＋ CIH group [ （0. 203 ± 0. 073 ）ukat/mg ], COX activity was higher than that in CIH group （P 〈 0. 05）, but still lower than that in NC group （ P 〈 0. 05 ）. Compared with NC group （ 47. 325 ± 7. 502）, decreased COX Ⅰ expression was observed in CIH group （ 10. 789 ± 8. 144, P 〈 0. 01 ） and E ＋CIH group （25. 593 ± 11. 108, P 〈0. 01）. While in the mean time, the protein content of COX Ⅰ in E ＋ CIH group was higher than that in CIH group （ P 〈 0. 05 ）. There was no significant difference in protein content of COXⅣ among the three groups （P 〉 0. 05 ）. COXI and COXⅣ mRNA levels had no significant change among the three groups （ P 〉 0. 05 ）. Conclusions CIH exposure could decrease mitochondrial
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2008年第10期604-607,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金（30572070）
上海市科学技术委员会重点基础项目（05JC14057）
