摘要
目的构建乙肝乙脑麻疹多表位噬菌体疫苗,并检测其免疫原性。方法将PCR方法合成的乙肝乙脑麻疹多表位基因COM插入T7噬菌体基因组中,使其与噬菌体10B蛋白融合,展示在T7噬菌体表面,构建成多表位噬菌体疫苗COM/T7。分别以1010pfu/只和1012pfu/只两种剂量,通过腹腔、皮下和鼻腔3种途径免疫昆明小鼠,ELISA检测小鼠特异性抗体水平。结果重组噬菌体COM/T7经PCR鉴定证明构建正确。乙肝乙脑麻疹多表位噬菌体疫苗能诱导小鼠产生针对各表位的特异性IgG抗体。在3种免疫途径中,腹腔免疫效果最好。免疫剂量与诱导的抗体水平在一定范围内呈正相关。多表位噬菌体疫苗诱导的抗-HBs和抗-JEV水平高于常规疫苗,而抗-MV水平低于常规疫苗。结论构建的乙肝乙脑麻疹多表位噬菌体疫苗具有良好的免疫原性。
Objective To construct a phage displayed polytope vaccine against hepatitis B （HB）, Japanese encephalitis （JE） and measles and study its immunogenicity. Methods Synthesize HB-JE-measle polytope gene COM by PCR and insert into the genome of T7 phage for fusion with phage 10B protein and display on the surface of T7 phage. Immunize KM mice with the constructed phage displayed polytope vaccine COM/T7 at dosages of 10^10 and 10^12 pfu by intraperitoneal, subcutaneous and intranasal routes separately and determine the specific antibody level by ELISA. Results PCR proved that recombinant phage COM/T7 was constructed correctly. The polytope vaccine induced specific IgG antibodies specific to various epitopes in mice. The IgG level induced by intraperitoneal route was higher than those by subcutaneous and intranasal routes. The antibody level induced was positively related to the dosage of vaccine within a certain range. Compared with those by routine vaccine, the anti-HBs and anti-JEV antibody levels induced by the polytope vaccine were high, while anti-MV antibody level was low. Conclusion The constructed phage displayed polytope vaccine against HB, JE and measles showed good immunogenicity.
出处
《中国生物制品学杂志》
CAS
CSCD
2008年第9期782-785,共4页
Chinese Journal of Biologicals
基金
国家863计划资助项目(2006AA02Z4C0)
云南省社会发展攻关项目(2006SG05)
关键词
乙型肝炎
乙型脑炎
麻疹
噬菌体展示
多表位疫苗
免疫原性
Hepatitis B
Japanese encephalitis
Measles
Phage display
Polytope vaccine
Immunogenicity
