摘要
目的构建重组血管内皮生长因子受体(VGFR)／中心实验激酶功能区受体(KDR)噬菌体疫苗并且观察其抗肿瘤效果。方法将人源VGFR、KDR的膜外部分插入到T7噬菌体的基因组中,作为一种融合蛋白展示在T7噬菌体外壳上,从而构建了一种新型的抗肿瘤疫苗。ELlSA方法检测抗VGFR、KDR 抗体,C57小鼠经过四次免疫,接种Lewis肺癌后记录肿瘤生长情况,评价疫苗效果。结果成功从人 A549细胞中可克隆出VGFR、KDR基因,并且构建了相应的噬菌体疫苗。ELISA方法检测到了免疫小鼠血清中有特异性抗体的产生,滴度可达104,肿瘤生长曲线显示噬菌体疫苗有明显的抑制肿瘤生长的作用。结论提示利用噬菌体展示技术构建的噬菌体疫苗的应用是一种非常有效的抗肿瘤方法。
Objective To construct the rccombined phage which was inserted the gene of homo VGFR,KDR as a new type vaccine and to evaluate the efficiency of the phage vaccine against the mice lewis lung cancer.Methods The T7 phage display system was applied for displaying the fragments of extra-cellular domain of homo KDR and the complete VGFR165. The specific antibody was detected by ELISA method.The phage vaccines were injected into the C57 mice 4 times before lewis lung cancer cells were inoculated. The tumor tissues were recorded to evaluate the anti-tumor effect of each vaccine. Results The phage vaccines inserted with the homo VGFR and the KDR gene were constructed successfully. The specific antibody was induced in the immuned mice compared with the control group. The tumor growth was inhibited in the experimental groups compared with the control one （P〈0.01, in KDR and VEGF groups）.Conclusion The phage displaying technology can be used as a new type vaccine against the tumor.
出处
《实用医药杂志》
2006年第5期586-589,共4页
Practical Journal of Medicine & Pharmacy
