摘要
目的为了完善现有的SIV/恒河猴模型,掌握恒河猴被SIV感染后体内细胞免疫应答状态,为评价HIV疫苗提供方法和数据上的参考,我们测定了SIV感染猴体内病毒特异性的细胞免疫水平。方法实验前选出4只无SIV、STLV、SRV/D和B病毒感染的恒河猴,用SIVmac239病毒液静脉感染实验猴,使用RT-PCR、流氏细胞术和ELISPOT等方法,监测SIVmac239病毒在恒河猴体内复制情况、感染猴的外周免疫损伤情况和细胞免疫情况,持续测定一年。结果实验结果显示IFN-γELISPOT方法能有效的评估实验猴的细胞免疫情况,IFN-γELISPOT结果和CD4+T细胞数无相关性,与血浆病毒载量稍有相关。结论本实验明确了SIVmac239感染中国恒河猴体内CTL的基本趋势和范围,了解了外周血病毒载量、外周免疫损伤与细胞免疫状况之间的联系,完善了SIV/SAIDS模型评价指标,为使用此模型评价抗病毒药物或疫苗提供了基础条件。
Objective To measure the cell mediated immunity much more accurately, the IFN-γ ELISPOT assay to assess the cell mediated immunity that was induced by virus or HIV vaccine were developed. Methods In the study,Four Chinese-origin rhesus macaques were used. First, all the monkeys were screened by serological test to ensure that they were free of BV,SIV,SRV, and STLV viruses. And then they were infected with SIVmac239. We analyzed results of Chinese-origin rhesus macaques by FACS, RT-PCR and ELISPOT detections. Results The result revealed the IFN-7 ELISPOT method could effectively test experiment monkey＇ s cellular immunity situation. Conclusion The study is clear about the basic tendency and the CTL of scope Chinese rhesus monkey infect with SIVmac239. Our finding provided the new basic set point for SIV/Chinese-origin rhesus macaques＇ model, and it has provided the foundation condition for used this model evaluate the medicine or vaccines against HIV-1.
出处
《中国比较医学杂志》
CAS
2008年第9期39-43,共5页
Chinese Journal of Comparative Medicine
基金
中央级公益性科研院所基本科研业务费专项资金
项目号DWS200712
