摘要
本文分别以沙眼衣原体（Ｃｈｌａｍｙｄｉａｔｒａｃｈｏｍａｔｉｓ，Ｃｔ）隐蔽性质粒引物（Ｐｐ）及主要外膜蛋白基因（ｏｍｐ；）引物（Ｐｍ）．用ＰＣＲ扩增Ｃｔ特异性ＤＮＡ，并用单链构象多态性（Ｓｉｎｇｌｅ－ｓｔｒａｎｄｃｏｎｆｏｒｍａｔｉｏｎｐｏｉｙｍｏｒｐｈ－ｉｓｍ，ＳＳＣＰ）作基因分型。４２６份标本中，９２例质粒ＰＣＲ阳性，用质粒ＰＣＲ阳性标本作ｏｍｐ：ＰＣＲ，６６例为阳性。其中５２例标本经ＳＳＣＰ分析显示７种不同的电泳图谱，１８例与标准株ＴＥ５５电泳图谱相同。其中两份标本分别取羊水及新生儿睑结膜，其ＳＳＣＰ图谱相同，表明两株Ｃｔ核酸序列完全相同。在分子水平上证实了Ｃｔ的垂直传播。临床标本可先采用敏感的质粒ＰＣＲ作初筛。阳性者再作ｏｍｐ１ＰＣＲ进行基因分型。应用ＰＣＲ－ＳＳＣＰ对Ｃｔ作基因分型，在分子流行病学研究中有重要意义。
Rapid diagnosis of chlamydia frachomatis infection was done by using PCR to amplify C. trachozoatis specific DNA with the cryptic plasmid primer and the major outer membrane protein gene(ompl)primer seperately, Of 426 samples, 92 were positive for plasmid PGR, Of plasmid PCR positive samples, 66 were positive for ompl PCR. 52 samples were analyaed by SSCP and 7 diferent profiles were detected. 18 samples showed the same profile compared with TE55 reference strain. An amniotic sample and a conjunctival saznple obtained from a neonate exhibited identical BSCP profile. lt indicated that the D NA sequences of the two C.trachomatis strain were identical. and vertical transmission of C. trachomatis was confirmed in the case. When genotyping was performed by using clinical apecimens, it was suitable to screen the samples by plasmid PGR and the positive specimens were further performed by ompl PCR for genotyping. Genotyping of C. trachomatis could be perfomed by PCR - SSCP and it was important in molecular epidemiological rescarch.
出处
《重庆医科大学学报》
CAS
CSCD
1997年第4期287-289,共3页
Journal of Chongqing Medical University
基金
国家自然科学基金
