摘要
研究不同木霉菌转化体对番茄灰霉病防治效果及机理,为木霉菌生物防治的合理利用奠定基础。利用限制性内切酶介导基因整合技术(restriction enzyme-mediated integration,REMI),通过插入线性化质粒DNA获得了生物防治番茄灰霉病(Botrytis cinerea)效果优于出发菌T21菌株(出发菌)的3个木霉菌转化体Ttrm31、Ttrm34和Ttrm55,对侵染花器和叶片的灰霉病防效分别比原生物防治木霉菌株提高了16.9%和8%。木霉菌转化体的产孢能力、分生孢子的萌发率、对碳氮源的利用能力及对高温的抵抗能力都有所提高;木霉菌转化体本身产生的几丁质酶和β-1,3-葡聚糖酶的活性均比出发菌高,因此通过REMI技术可以获得新的有益木霉菌转化体,在一定程度上提高了生物菌株防治番茄灰霉病的水平。说明REMI技术可以用于改良生防木霉菌株的功能,提高生物防治效果。
Control of tomato gray mold by Trichoderma REMI strains and the mechanism were studied for the rational application of Trichoderrna to pest control. The mutant Trichoderma strains Ttrm31, Ttrm34 and Ttrm55 were obtained from original Trichoderma strain T21 by REMI（restrietive enzyme-mediated integration）technique for more effective biocontrol of the tomato gray mold, Botrytis cinerea. The insertional mutagenesis improved Trichoderma bioeontrol efficacy against tomato gray mold in vitro and in vivo. The mutants enhanced control of gray mold on flowers and leaves by 16.9% and 8%, as compared with original biocontrol strain of Trichoderma, respectively. The results suggested that these mutants had higher ability of spore-production, spore-germination, usage of carbon and nitrogen and resistance to high temperature. At the same time, these mutants could produce ehitinase and β-1,3-glueanase of higher activities in vitro and also induced higher production of the two enzymes. Taken together, making mutation of Trichoderma strains by REMI could be one way to develop new biocontrol agents against plant diseases.
出处
《植物保护》
CAS
CSCD
北大核心
2008年第5期80-85,共6页
Plant Protection
基金
教育部"948"项目(2010056)
辽宁省博士启动项目(20051051)
辽宁省教育厅课题(20040323)
