摘要
采用酸性聚丙烯酰胺凝胶电泳技术（A-PAGE）对采自中国四川、重庆、甘肃、日本5个地区的42份野生鹅观草材料进行遗传多样性分析，获得下述结果，1）供试材料共分离出29条带纹，多态率达89．66％。材料间的遗传相似系数范围在0．448-0．966，平均GS值为0．695，说明供试野生鹅观草具有较为丰富的遗传多样性。2）对所有材料进行聚类分析，可聚为3类，基本能按照生态地理环境来源聚为一类，表明供试材料的聚类和其生态地理环境间有一定的相关性。3）基于Shannon多样性指数估算了5个鹅观草生态地理类群内和类群间的遗传分化，发现类群内遗传变异占总变异的83．69％，类群间遗传变异占总变异的16．31％。4）对各生态地理类群基于Nei氏无偏估计的遗传一致度聚类分析表明，各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性。
Acid polyacrylamide gel electrophoresis （A-PAGE） molecular markers were used to detect the genetic diversity among 42 wild accessions of Roegneria learnoji collected from four regions （Sichuan, Chongqing,Gansu and Japan）. 1） A total of 29 bands of which 89.66 percent were polymorphic were detected in all accessions. The Nei genetic similarity coefficients of the tested accessions ranged from 0. 448 to 0. 966, with an average Nei of 0. 695. These results suggested that there was rich genetic diversity among the tested wild resources of R. kamoji. 2） 42 wild accessions could be clustered into three groups. Moreover, the groups from the same origin frequently clustered into one group suggesting a correlation among the tested wild resources, geographical and ecological environment. 3） Genetic differentiation between and within five eco-geographical groups of R. kamoji was estimated by Shannon＇s diversity index, which showed that 83.69 percent genetic variance existed within groups, and 16.31 percent genetic variance was between groups. 4） UPGMA cluster analysis based on Nei＇s unbiased measures of genetic identity was assayed for six eco-geographical groups of R. kamoji, indicating that there was a correlation between genetic differentiation and eco-geographical habits between the five groups.
出处
《草业学报》
CSCD
2008年第5期138-144,共7页
Acta Prataculturae Sinica
基金
南方优良饲草选育与分子聚合育种体系的基础研究
科技部973项目(2007CB108907)
四川省科技厅"十一五"牧草育种攻关项目(02SG023-001)
农业部草地农业生态系统学重点开放实验室资助
关键词
鹅观草
醇溶蛋白
遗传多样性
聚类分析
Roegneria kamoji
gliadin
genetic diversity
cluster analysis
