摘要
为检测二羟基乙二肟（ＤＨＧ）对细胞的恶性转化能力，判断其体外致癌性，本研究用ＤＨＧ对ＢＡＬＢ／ｃ－３Ｔ３细胞进行转化试验。结果表明：ＤＨＧ能诱导ＢＡＬＢ／ｃ－３Ｔ３细胞发生转化，转化细胞可使刀豆球蛋白（ＣｏｎＡ）凝集并可在软琼脂上生长，将其接种于ＢＡＬＢ／ｃ裸小鼠皮下能诱发出纤维肉瘤。以上结果充分说明ＤＨＧ诱导的ＢＡＬＢ／ｃ－３Ｔ３细胞的转化为恶性转化。
BALB/c3T3 cell transformation test was used to detect cell carcinogenicity caused by dihydroxyglyoxime (DHG) and determine its carcinogenicity in vitro. Results showed that DHG could induce transformation of BALB/c3T3 cells and the transformed cells could agglutinate in the presence of Con A and grew in the soft agar. Transformed cells could induce the formation of fibrosarcoma if they were inoculated subcutaneously into the naked mice. It indicated sufficiently that the transformation in BALB/c3T3 cell culture was malignant, and also predicted its potential carcinogenicity in human beings.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1997年第6期246-248,共3页
Chinese Journal of Preventive Medicine
