摘要
采用紫外线诱变法对6株特效菌进行处理,考察了诱变前后菌株理化性能及对难降解底物去除能力的变化.结果表明,紫外线诱变使菌株形态和ERIC-PCR指纹图谱发生了明显改变;诱变后的菌株对目标难降解底物的降解能力均得到改善,其中,PNCB3、CB4、14357、EM的降解率提高了20%以上.诱变后菌株经7代转接后,降解性能无显著降低,具有一定遗传稳定性.诱变后复合菌剂可以明显提高废水生化处理系统对难降解物质的CODCr的去除速率,延滞期缩短近2h,速率常数增大1.68倍,同时可以显著提高系统抗击负荷与有毒物冲击的能力.
Six high efficient microbes were mutated by ultraviolet mutagenesis. The physiochemical properties and refractory substance removal ability of the microbes before and after mutation were compared. Obvious change in microbial shape and ERIC-PCR finger print were observed after mutation; The target refractory substrate degradation ability of microbes was improved, the degradation rate of PNCB3, CB4, 14357, EM was improved more than 20%. Submerged cultures showed that the hereditary character of high degradation ability of mutant microbes was stable; the mixed microbial agent after mutation can improve the CODcr degradation rate of biological wastewater treatment system significantly. The lag phase was shortened 2h and the velocity coefficient increased by 1.68 times in the system. Meanwhile, the load shock and toxic substance shock resisting ability were improved greatly.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2008年第9期807-812,共6页
China Environmental Science
基金
国家自然科学基金资助项目(50678165)
中国科学院知识创新项目(KSCX2-YW-G-008)
关键词
生物强化
紫外诱变
菌剂
难降解有机物
bioaugmentation
ultraviolet mutagenesis
microorganisms agent
refractory organicpollutants
