期刊文献+

制药废水处理系统微生物群落动态变化的ERIC-PCR指纹图谱分析 被引量:8

Analysis of the Microbial Community of Activated Sludge in a Treatment System of Medicine Wastewater From Pharmaceuticals Production by ERIC-PCR Fringerprinting
下载PDF
导出
摘要 用ERIC-PCR指纹图谱监测制药综合废水活性污泥生态系统,结合活性污泥性质测定和理化指标监测结果,综合评价处理系统运行状态.4个时间点的监测中,受高盐影响污泥沉降比和污泥指数略微偏高,第1监测时间最高分别为68%和282.93 mL/g,第4监测时间最低分别为39%和187.64 mL/g;CODCr去除率在73.23%~86.73%之间;ERIC-PCR指纹图谱的多样性指数稳定在2.24~2.38之间,相似性指数差异较大,最高为95%,最低为19%.表明第1监测时间高负荷冲击会导致系统CODCr去除率下降,第2监测时间短时期缺氧不会影响系统正常运行,第3、4监测时间水质水量稳定活性污泥处理系统微生物种群结构和处理效率稳定. The occurrence, settleability and activity of sludge in a treatment system of medicine wastewater from pharmaceuticals production were estimate by ERIC -PCR (Enterbacterial Repetitive Intergenic Consensus - PCR) fingerprinting combined with activated sludge characterization ( SV30, SVI) and CODer measurements. During the monitoring period, high - sanlinity wastewater caused SV30 and SVI appreciably higher than natural level,the highest at M1 with 68%and 282. 93mL/g and lowest at M4 with 39%andl 87.64ml/g, the removal efficiency of CODer fluctuated from 73.23% to 86.73%. ERIC - PCR banding patterns of activated sludge samples were fairly stable between 2.24 and 2.38 for Shannon - weaver indices, and greatly fluctuant from 19% between M1 and M2 to 95% between M3 and M4. The results indicate that the presence of a pollution shock causes low removal efficiency of CODer at M1, short - time anxic condition doesit impact treatment system of wasterwater at M2 ,and stabilization of wastewater quality and quantity serve as the relatively stable removal efficiency of CODer and microbial community of activated sludge between M3 and M4.
出处 《辽宁大学学报(自然科学版)》 CAS 2008年第2期158-161,共4页 Journal of Liaoning University:Natural Sciences Edition
基金 辽宁省科技厅攻关项目(2005229003 2006229001)
关键词 制药废水 微生物群落 ERIC-PCR medicine wastewater bacterial community structure EERIC - PCR.
  • 相关文献

参考文献10

  • 1祁佩时,陈战利,于桂清,刘云芝,赵月龙.复合生物法处理难降解制药废水的研究[J].中国环保产业,2005(10):31-33. 被引量:7
  • 2高平平,赵义,赵立平.焦化废水处理系统微生物群落结构动态的ERIC-PCR指纹图谱分析[J].环境科学学报,2003,23(6):705-710. 被引量:15
  • 3陈敏,魏桂芳,高平平,王凌华,庞晓燕,赵立平.用ERIC-PCR结合分子杂交监测焦化废水处理系统(A^2/O)中微生物群落结构的变化[J].生态学报,2004,24(7):1330-1334. 被引量:8
  • 4Huhon C S, C F Higgins, P M Sharp. ERIC sequences: a novel family of repetitive elements in the genomes of Escherichia coli, Salmonella typhimurium and other enterobacteria [ J ]. Mol Microbiol, 1991, 5 : 825 - 34. 被引量:1
  • 5Di Giovanni G D, Watrud, L S, Seidler R J, et al. Fingerprinting of mixed bacterial strains and BIOLOG Gramnegative (GN) substrate communities by enterobacterial repetitive intergenic consensus sequence -PCR (ERIC -PCR) [ J ]. Curr Microbiol , 1999, 38, 217-223. 被引量:1
  • 6Di Giovanni G D, L S Watrud, R J Seidler, et al. Comparison of Parental and Transgenic Alfalfa Rhizosphere Bacterial Communities Using Biolog GN Metabolic Fingerprinting and Enterobacterial Repetitive Intergenic Consensus Sequence - PCR ( ERIC - PCR) [J]. Microb. Ecol. 1999,37:129-139. 被引量:1
  • 7国家环保局.水和废水监测分析方法[M].北京中国环境科学出版社,2003. 被引量:1
  • 8Sambrook J Maniatis T Maniatis T.分子克隆实验指南[M].北京:科学出版社,1992. 被引量:6
  • 9Versalovic, J. , T. Koeuth, and J. R. Lupski. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes [ J ]. Nucleic Acids Res, 1991 , 19:6823 - 31. 被引量:1
  • 10Murray A E, Hollibaugh J T, Orrego C. Phylogenetic composition ofbacterioplankton from two Califorhia estuaries compared by denaturing gradient gel electrophoresis of 16Sr DNA fragments [J]. Appl Enriron Microbiol, 1996,62 (7) :2676 - 2680. 被引量:1

二级参考文献7

共引文献32

同被引文献100

引证文献8

二级引证文献35

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部