摘要
目的:观察不同浓度五倍子水提取物对内毒素诱导人牙髓细胞分泌IL-6的影响。方法:采用组织块法体外培养人牙髓细胞,以含不同浓度五倍子水提取物(1.25、2.5、5、10、20μg/mL)、25μg/mL内毒素和20mL/L新生牛血清的DMEM培养基作用于第5代人牙髓细胞,用放射免疫法测定IL-6含量,采用单因素方差分析(完全随机设计)和t检验对实验数据进行统计学分析。结果:低浓度(1.25、2.5、5、10、20μg/mL)五倍子水提取物能明显抑制25μg/mL内毒素诱导人牙髓细胞分泌IL-6,这种抑制作用在一定范围内呈浓度依赖性。结论:低浓度五倍子水提取物具有抑制内毒素诱导人牙髓细胞分泌IL-6的作用。
AIM： To observe the influence of Chinese nutgall extracts in different doses on the effect of lipopolysaccharide upon the secretion of IL -6 from human dental pulp cells. METHODS： Human dental pulp cells were cultivated with tissue explant. Human dental pulp cells of the fifth passage were cultured in DMEM with 25 μg/ml lipopolysaccharide, Chinese nutgall extracts（ 1.25,2.5,5,10, 20 μg/ml） and 20ml/L new born bovine serum. Radioimmunoassay （RIA） was applied to determine the amount of cells＇ IL- 6. One -way ANOVA（ completely random design） and t - test were used in statistical analysis. RESULTS ： Low - dose of Chinese nutgall extracts （ 1.25,2.5,5, 10,20 μg/ml）significantly inhibited the effect of lipopolysaccharide on the human dental pulp cells＇secretion of IL -6 in a dose - dependent manner. CONCLUSION： Low - dose Chinese nutgall extracts can inhibit the effect of lipopolysaccharide on the secretion of IL -6 from human dental pulp cells .
出处
《牙体牙髓牙周病学杂志》
CAS
2008年第9期518-520,共3页
Chinese Journal of Conservative Dentistry
关键词
五倍子
内毒素
牙髓细胞
IL-6
Chinese nutgall
lipopolysaccharide（LPS）
dental pulp cell
IL - 6
