摘要
目的:本研究旨在通过对临床可能(可疑)克雅病(CJD)病例及其他神经系统病变患者的150份脑脊液标本进行14—3-3蛋白检测,对其临床意义和临床应用价值进行初步探讨。方法:用Western blot方法检测脑脊液标本中的14-3-3蛋白。结果:150份标本中共19例阳性,其中临床诊断CJD者(n=6),5例阳性;临床疑似CJD者(n=23),6例阳性;非CJD痴呆者(n=6),1例阳性;单纯疱疹病毒性脑炎(n=7),3例阳性;其他病毒性脑膜脑炎、结核性脑膜脑炎、新型隐球菌脑膜炎、脑梗死各有1例阳性。结论:在临床诊断及疑似CJD病例中,脑脊液14-3-3蛋白检测是重要的实验室诊断手段;脑脊液14-3—3蛋白在CJD的诊断中强调病例的选择性。
Aim： To evaluate clinical significance of 14-3-3 protein detection in diagnosis of Creutzfeldt- Jacob disease （CJD） and other disorders of central nervous system（CNS）. Methods： 150 cerebrospinal fluid （CSF） samples were detected with Western blot. 26 samples of them were from cases with probable CJD （n=6） and possible CJD （n=23）, clinically. Results： In all samples, 19 samples showed a positive test. In 5 of 6 cases with probable CJD and 6 of 23 possible CJD were positive. Positive test was found in 1 of 6 patients with non-CJD dementia and in 3 of 7 patients with herpes simplex virus encephalitis. Positive results were also found in other disorders in CNS, including undefinite virus encephalitis（1）, tuberculous meningitis and encephalitis （1）, cryptococcosis（1） and cerebral infarction（1）. Conclusion： Detection of 14-3-3 protein in CSF is one of the most important tests to establish clinical diagnosis of CJD, the valuation of 14-3-3 protein in diagnosis of CJD is associated with the choice of the case.
出处
《中国临床神经科学》
2008年第5期490-494,共5页
Chinese Journal of Clinical Neurosciences
关键词
克雅病
14-3-3蛋白
脑脊液
Creutzfeldt-Jacob disease
14-3-3 protein
cerebrospinal fluid
