摘要
目的探讨转录因子FOXO1和FOXO3a在系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中的表达水平及其与SLE疾病活动性的关系。方法选择SLE活动性、SLE非活动性患者和正常对照，运用反转录-聚合酶链反应（RT-PCR）和westem blot检测SLE患者和健康对照组PBMC中FOXO1和FOXO3a mRNA及蛋白的表达水平。结果活动性SLE患者PBMC中FOX01 mRNA和蛋白的表达水平明显低于SLE非活动性患者和正常对照（P〈0．05），非活动性SLE患者F0XO1 mRNA和蛋白的表达水平明显低于正常对照（P〈0．05）。F0XO3a mRNA和蛋白的表达水平在3组间差异无统计学意义。FOXO1 mRNA水平与狼疮活动指数（SLEDAL）呈显著负相关（r=-0.651，P〈0.05）。结论SLE患者PBMC中FOX01 mRNA和蛋自的表达水平显著低于健康对照，随着SIE患者病情的好转，FOXO1 mRNA和蛋白的表达水平逐渐增高。FOXO1与SLE疾病的活动性呈负相关。FOXO3a与SLE疾病的活动性无关。
Objective To study the expression of transcription factor FOXO1 and FOXO3a on peripheral blood mononuclear cells （ PBMC ） in patients with active or inactive systemic lupus erythematosus （SLE） and investigate the effect of FOXOI and FOXO3a on the clinical features of SLE. Methods Thirty SLE patients and 10 healthy controls were enrolled. PBMC were separated from the peripheral blood.Reverse transcription-polymerase chain reaction （RT-PCR） and western blot were applied to analyze the expression of FOXO1 and FOXO3a. Results The level of FOXO1 expression was significantly decreased in active SLE patients compared with controls and patients with inactive SLE （P〈0.05）. The level of FOXO1 expression in inactive SLE patients was lower than that of the controls （P〈0.05）. The expression of FOXO1 mRNA was negatively correlated to SLEDAI. However, the level of FOXO3a was similar among the three groups. Conclusion The result suggests that FOXO1 may be involved in the pathogenesis of SLE and the expression level of FOXO1 may be a good indicator for the disease activity of SLE.
出处
《中华风湿病学杂志》
CAS
CSCD
2008年第9期629-631,共3页
Chinese Journal of Rheumatology
