摘要
目的:通过检测角膜烧伤后前房水中TGF-β2含量的变化,探讨在眼部内环境下TGF-β2与角膜烧伤之间的关系,为角膜烧伤治疗提供依据。方法:制备Wistar大鼠角膜碱、酸、热中度烧伤动物模型,分别于伤后4,8h;1,3,5,7,14,28,49d处死。采集房水:用20g/LBSA包被的毛细管于角膜缘处刺入角膜,吸取房水至20g/LBSA包被的离心管中,-70℃保存。标准品和样品进行酸激活处理。使用ELISA方法检测角膜烧伤后前房水中TGF-β2的水平。结果:大鼠角膜碱烧伤后4hTGF-β2的水平呈现一过性升高,然后下降,3d时TGF-β2含量再次升高,7d时达高峰,持续至7wk时降至正常。大鼠角膜酸烧伤后4hTGF-β2的水平呈现一过性升高,然后下降,低于正常对照组,两者差异显著。7d时房水中TGF-β2升至正常,然后一直保持低平状态。49d时我们检测房水中TGF-β2水平仍低于正常。大鼠角膜热烧伤后4hTGF-β2的水平呈现一过性升高,1d时降至最低,3d时开始增长,直到7d时我们检测仍高于正常,然后缓慢下降。结论:角膜烧伤后前房水中TGF-β2的含量发生变化,致伤原因不同,其变化方式不同。角膜碱烧伤和热烧伤后于7d时出现TGF-β2高峰,说明此时角膜处于免疫功能抑制状态,这对于维持角膜前房的免疫赦免状态非常重要,证明此时机体发挥了自我保护功能,避免产生过度的病理反应。角膜酸烧伤后TGF-β2水平始终低平,其原因尚有待于进一步研究。
AIM- To study the relationship between TGF-β2 and ocular burns by detecting the levels of TGF-β2 in rat aqueous humor following corneal alkali burns, acid burns and thermal burns and to provide experimental clues. METHODS. The models of alkali burns, acid burns and thermal burns in Wistar rats were established and the animals were sacrificed at 4, 8 hours, 1, 3 day, 1, 2, 4 and 7 weeks after burn. Aqueous humor was obtained by the anterior chamber paracentesis and frozen at -70℃. 1mol/L HCI was added to the samples to acid activation. The levels of TGF-β2 were assessed with a specific-capture ELISA kit. RESULTS： The levels of TGF-β2 in rat aqueous humor following corneal alkali burns increased at 4 hours after injury and decreased quickly. The second increase was appeared at 3 days, reached to the peak at 1 week and came back to the normal at 7 weeks. The levels of TGF-β2 in rat aqueous humor following corneal acid burns increased at 4 hours after injury and decreased quickly, There was significant difference compared to the control,The levels of TGF-β2 increased to the normal at 1 week and lasted low expression as far as 7 weeks. The levels of TGF-β2 in rat aqueous humor following corneal thermal burns increased at 4 hours after injury and decreased quickly to the lowest expression at 1 day. We observed the second increase at 3 days and it lasted higher expression for long time. CONCLUSION： The levels of TGF-β2 in rat aqueous humor changes following ocular burns. There is difference among different burns. The levels of TGF-β2＇s peak appears respectively at 1 week following alkali burns and thermal burns show the cornea be in immunosup- pressive state this time and it is very important to maintain the immune privileged status of the anterior chamber. It is proved that the self-safeguard of the body avoids excessive pathological reaction. The reason of low levels of TGF-β2 following acid burns needs more study.
出处
《国际眼科杂志》
CAS
2008年第9期1819-1822,共4页
International Eye Science
基金
中国黑龙江省青年科学基金(No. QC05C42)~~
