摘要
目的:探讨TGF-β2反义寡核苷酸对兔角膜基质成纤维细胞转化、增殖的作用,揭示其对角膜创伤修复的影响。方法:新西兰大白兔28只,双眼均制备角膜基质创伤模型,右眼为实验组,用浸有TGF-β2反义寡核苷酸的8-0薇乔缝线缝合角膜创口;左眼为对照组,用普通8-0薇乔缝线缝合角膜创口,分别于4,7,14,21d处死动物,取角膜后行免疫组化(α-SMA和PCNA)染色和电镜观察。结果:术后各时间段均发现,实验组α-SMA和PCNA阳性的成纤维细胞数明显少于对照组,但成纤维细胞的超微结构实验组和对照组比较无明显区别。结论:TGF-β2反义寡核苷酸可抑制兔角膜基质成纤维细胞转化、增殖,为调控角膜基质伤口修复提供了一个新的途径。
AIM： To investigate the influence of TGF-β2 antisense oligonucleotide（ASON） on the differentiation, proliferation of stromal fibroblast in corneal stroma injury.
METHODS： Corneal stroma injury was performed on both eyes of 28 rabbits, the right eyes were served as experimental group, corneal incisions were sutured with 8-0 coated vicryl suture carrying TGF-β2 ASON; the left eyes were served as control group, corneal incision were sutured with common 8-0 coated vicryl suture. Rabbits were killed at 4, 7, 14 and 28d after surgery, stromal flbroblasts were examined by immune histochemistry and electron microscopy.
RESULTS： At different time points, TGF-β 2 ASON significantly reduced the numbers of cells expressinga -smooth muscle actin （α -SHA） and proliferating cell nuclear antigen （PCNA）. The ultrastructure of fibroblast had no significant difference in two groups.
CONCLUSION： TGFβ2 ASON can inhibit the differentiation and proliferation of stromal fibroblast in corneal injury. This will be a new method to adjust corneal wound repair.
出处
《国际眼科杂志》
CAS
2006年第2期291-294,共4页
International Eye Science
基金
中国深圳市科技局资助项目(No.2002104)~~
关键词
反义寡核苷酸
缝线
角膜
瘢痕
TGF-β2
antisense therapy
corneal repair
Earring
