摘要
目的观察急性肾缺血/再灌注(I/R)热休克蛋白70(HSP70)的表达规律,及中药血必净注射液对肾I/R损伤的保护作用机制,探索药物诱导机体产生HSP70的可行性。方法普通级雌性SD大鼠108只,体重(200±20)g,随机分为3组:正常对照组(A组);模型组(B组)于手术前5～10 min从股静脉注射生理盐水5 ml/kg;活血化瘀中药组(C组)于手术前5～10 min从股静脉注射血必净注射液5 ml/kg,各组再分为再灌注0、6、12、24、36和48 h 6个亚组,每个亚组6只。于各时间点取血测血肌酐(SCr)、尿素氮(BUN);一侧肾组织用蛋白质免疫印迹法(Western blotting)检测HSP70表达;另一侧肾组织用于苏木素-伊红(HE)染色及免疫组化检测。结果B组与C组SCr和BUN于再灌注6h起均显著高于A组,C组低于B组,差异有统计学意义(P<0.05或P<0.01),表明血必净注射液有对抗肾I/R损伤并保护肾功能的作用。Western blotting扫描及免疫组化结果显示,C组HSP70表达在再灌注12h较B组提前达到高峰,表明血必净注射液诱导HSP70表达提前达高峰,发挥保护作用。结论血必净注射液通过刺激HSP70提前表达并显著升高,引发了内源性肾脏保护机制,为临床上早期预防急性肾功能衰竭发生和寻找肾I/R损伤的药物治疗提供重要的理论依据。
Objective To explore the regularity of heat shock protein 70 （HSP70） expression and its function in renal ischemia/reperfusion injury （IRI）, and to reveal whether traditional Chinese medicine, Xuebijing injection （血必净注射液）, induces HSP70 synthesis and has a protective effect on IRI. Methods One hundred and eight female Sprague-Dawley （SD） rats, （200 ± 20） g in weight, were used in this experiment, and randomly divided into three groups （each n= 36）; control （A）, model （B） and treatment group （C）. The I/R model was established by clamping renal pedicles on both sides for 45 minutes to cause ischemia and then reperfusion was made. In group A, a similar model procedure was performed, but without ischemia. At 5 -10 minutes before the IRI was performed, 5 ml/kg of normal saline and 5 ml/kg of Xuebijing injection was injected through the femoral vein in group B and C respectively. In B and C groups, according to the durations of reperfusion for 0, 6, 12, 24, 36 and 48 hours, the rats were subdivided into six subgroups （each n= 6）. At the end of each time point, blood and renal tissue were collected to measure blood creatinine （SCr） and urea nitrogen （BUN）, and Western blotting method was used to examine the expression of HSP70. In another kidney, renal tissue was obtained for hematoxylin and eosin （HE） staining pathological and immunohistochemical examinations. Results In B and C groups, the SCr and BUN levels at 6 hours after I/R were significantly higher than those in the A group, and those in the C group were lower than those in the B group （P〈0.05 or P〈0.01）, showing that Xuebijing injection has the action of anti-renal IRI and protective effect on renal functions. Western blotting scanning and immunohistochemical results revealed that the peak time of HSP70 expression in group B and C was at 24 hours and 12 hours respectively after reperfusion, indicating that Xuebijing injection could induce HSP70 expression in advance to reach peak level to
出处
《中国中西医结合急救杂志》
CAS
2008年第5期293-295,F0003,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
关键词
缺血/再灌注损伤
肾
热休克蛋白70
活血化瘀中药
肾脏
renal ischemia/reperfusion injury
heat shock protein 70
traditional Chinese medicine for promoting blood circulation and removing blood stasis
kidney
