摘要
目的观察六味地黄丸对TNF-α、IL-6表达水平的影响及糖尿病肾病大鼠肾脏的保护机制的研究。方法复制DN大鼠模型,将其分为正常组、模型组、治疗组(六味地黄丸高、中、低剂量组及罗格列酮组),全自动生化分析仪测大鼠肾功能、考马斯亮蓝法测24 h尿蛋白量情况,Elisa法检测血清中TNF-α、IL-6的水平,同时用葡萄糖氧化酶法测定空腹血糖(FBG)。结果六味地黄丸高、中剂量组及罗格列酮组大鼠24 h尿蛋白量、血Scr、BUN均低于模型组(P<0.01),且高剂量组疗效与与罗格列酮组差异无统计学意义(P>0.05);同时各治疗组TNF-α表达水平较模型组下调(P<0.01),其中六味地黄丸高剂量组与罗格列酮组之间差异无统计学意义(P>0.05);而六味地黄丸高、中剂量组和罗格列酮组IL-6在肾脏的表达均高于模型组(P<0.01),且高剂量组和罗格列酮缬组在表达上差异无统计学意义(P>0.05)。结论六味地黄丸可用于治疗早期糖尿病肾病,对肾脏有保护作用,机制可能与其能抑制DN大鼠肾脏中TNF-α、IL-6的激活有关。
Objective to observe the expression of Liuwei Dihuang Pill protection mechanism of kidney levels and diabetic nephropathy rats on TNF - α, IL -6. Methods the rat model of DN replication, which is divided into the normal group, model group, treatment group （Liuwei Dihuang Pill high, middle and low dose group, rosiglitazone group）, full automatic biochemical analyzer to measure renal function in rats with Coomassie brilliant blue method, 24 h urinary protein, serum TNF - α, Elisa method the level of IL -6, at the same time, fasting blood glucose was measured with glucose oxidase method （FBG）. Results of the Liuwei Dihuang Pill, 24 h urinary protein in high dose group and the rosiglitazone group rats, blood Scr, BUN were lower than those in the model group （ P 〈0. 01 ）, without statistical significance and effect in high dose group and the resiglitazone group （ P 〉 0.05） ; and the expression of TNF - α level in treatment group than those in the model group reduced （ P 〈 0.01 ） among them, there were no significant differences between the Liuwei Dihuang pill high dose group and the rosiglitazone group （ P 〉 0.05） ; and the Liuwei Dihuang Pill high, middledose group and rosiglitazone group, the expression of IL -6 in kidney were higher than those in the model group （ P 〈 0. 01 ）, and high dose group and Roger rosiglitazone valerian group there was no significant difference in the expres- sion （ P 〉 0.05）. Conchmion the Liuwei Dihuang pill can be used for the treatment of early diabetic nephropathy, and has a protective effect on the kidney, the mechanism may be related to the inhibition of the DN rat kidney of TNF -αt, IL - 6 activation.
出处
《现代中医药》
CAS
2014年第1期74-77,共4页
Modern Chinese Medicine
