摘要
目的探讨血必净注射液联合地塞米松防治大鼠百草枯中毒慢性肺损伤的作用及可能机制。方法30只雄性Wistar大鼠被随机均分为6组,每组5只。腹腔注射质量分数为20%的百草枯80 mg/kg制备大鼠中毒模型;正常对照组予生理盐水。染毒后2h,治疗组分别向腹腔注射小剂量血必净注射液(1.25 g/kg)、大剂量血必净注射液(2.50 g/kg)、地塞米松(25 mg/kg)及大剂量血必净注射液联合地塞米松(联合治疗),正常对照组和模型组给予等量生理盐水,均每日1次,连用4 d。观察各组大鼠染毒后反应;于28 d处死各组大鼠取相应标本,检测肺系数、肺匀浆中羟脯氨酸(HYP)、血清转化生长因子-β1(TGF-β1),同时行肺组织病理学观察。结果各治疗组血清TGF-β1、肺HYP均较模型组显著降低,以联合治疗组最为明显,血必净大剂量组和地塞米松组其次,血必净小剂量组仅TGF-β1好于模型组,差异有统计学意义(P均<0.05)。比较各组肺系数,联合治疗组最佳,其他治疗组间差异无统计学意义。肺组织病理学观察显示,治疗组肺纤维化及实变程度较模型组轻,其中联合治疗组最轻。结论血必净注射液联合地塞米松治疗百草枯中毒,通过抑制TGF-β1水平,减少纤维母细胞迁移、活化,抑制胶原蛋白产生,保护肺组织结构,可以更有效地抑制肺纤维化、肺实变。
Objective To explore the possible mechanism and protective effect of Xuebijing injection （血必净注射液） and dexamethasone on rats with paraquat-indueed chronic pulmonary injury. Methods Thirty male Wistar rats were randomly divided into six groups： normal group （n = 5）, model group （n = 5）, treatment groups （n=20）. In the normal group, normal saline was used, while in the other groups, 20% paraquat 80 mg/kg was injected peritoneally for poisoning. After 2 hours of intoxication, low dose Xuebijing injection （1.25 g/kg）, high dose Xuebijing injection （2.50 g/kg）, dexamethasone （25 mg/kg）, high dose Xuebijing injection combined with dexamethasone （combined group） respectively were administered into the four different treatment groups, equal amount of normal saline was given to the normal and model groups, and the treatment continued for 4 days. At 28 days after paraquat injection, 5 rats in each group were killed respectively, serum transforming growth factor-β1 （TGF-β1） and hydroxyproline （HYP） level in the lung homogenate were measured, and pulmonary coefficient and histological changes were observed. Results In the treatment groups, the levels of serum TGF-β1 and lung tissue HYP, pulmonary coefficient were less than those of model group, and among the treatment groups, combined group had the best results （all P〈0. 05）. There was no significant difference between the high dose Xuebijing group and dexamethasone group. In the low dose Xuebijing group, only TGF-β1 was better than that of model group, and the difference was significant （P〈0. 05）. When pulmonary coefficients were compared, the combined group was the best among the treatment groups, and no other statistical differences were found among the other treatment groups. The lung tissue pathological investigation showed that the pulmonary fibrosis and consolidation in the treatment groups were milder than those in the model group, and among them the combined group was the mildest. Conclusion Xuebij
出处
《中国中西医结合急救杂志》
CAS
2008年第5期282-285,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
天津市科技创新专项基金资助(06F22DSH00403)
