摘要
目的:研究RASSF1A蛋白和CyclinD1在胃癌及癌前病变组织中的表达及二者的关系.方法:收集延安大学附属医院2005-01/2006-10行手术切除的69例胃癌患者临床病理资料和切除标本的组织蜡块.同时收集慢性浅表性胃炎45例、慢性萎缩性胃炎45例、慢性胃炎伴肠化生45例及慢性胃炎伴不典型增生57例胃黏膜组织蜡块.采用免疫组化SP法检测胃癌及癌前病变胃黏膜组织中RASSF1A蛋白和CyclinD1的表达.结果:胃癌组织中RASSF1A表达的阳性率低于其在慢性浅表性胃炎胃黏膜组织中的阳性表达率(χ2=10.4,P<0.05);RASSF1A蛋白的在胃癌癌前病变黏膜中阳性率表达逐渐降低(χ2=28.7,P<0.05),并且与胃癌组织分化程度有关(P<0.05).胃癌组织中CyclinD1表达的阳性率为84.1%,慢性浅表性胃炎黏膜组织中的几乎不表达;CyclinD1在胃癌癌前病变黏膜中阳性率表达逐渐升高(χ2=11.9,P<0.05);胃癌组与其他各组之间阳性率比较均存在显著性差异(χ2=22.0,19.6,29.4,P<0.01);RASSF1A蛋白和CyclinD1蛋白在胃癌中阳性表达率二者呈负相关(rs=-0.323,P<0.05).结论:胃黏膜在从慢性浅表性胃炎→慢性萎缩性胃炎→肠化生→不典型增生→胃癌这一演化模式过程中,RASSF1A蛋白表达减弱,CyclinD1蛋白表达增强;二者的联合检测,对研究胃癌发生发展机制有一定科学意义.
AIM： To investigate the expression of Ras association domain family 1A gene （RASSFIA） and CyclinD1 as well as their correlations in gastric mucosal lesions. METHODS： We detected the expression of RASSFIA and CyclinD1 in tissue specimens of gastric cancer （n = 69）, chronic superficial gastritis （n = 45）, chronic atrophic gastritis （n = 45）, chronic gastritis accompanied with intestinal metaplasia （n = 45） and chronic gastritis accompanied with atypical hyperplasia （n = 47） using SP immunohistochemistry RESULTS： The positive rate of RASSF1A expression was significantly lower in gastric carcinoma than that in chronic superficial gastritis （x^2 = 10.4, P 〈 0.05）; In the mucosa of precancerous lesions, the expression of RASSFIA protein was decreased gradually （x^2= 28.7, P 〈 0.05）, and obviously correlated with the degrees of tumor differentiation （P 〈 0.05）. CyclinD1 protein was hardly expressed in chronic superficial gastritis, but its expression was significantly elevated in gastric cancer （84.1%）. The expression of CyclinD1 was increased gradually in the mucosa of precancerous lesions （x^2 = 11.9, P 〈 0.05）, and it was markedly higher in gastric carcinoma than that in the other lesions （x^2 = 22.0, 19.6, 29.4; all P 〈 0.01）. There was a negative correlation between the expression of RASSFIA and CyclinD1 protein in gastric carcinoma （rs = -0.323, P 〈 0.05）. CONCLUSION： RASSFIA expression is downregulated while CyclinD1 expression is upregulated in gastric mucosa during the evolution process of chronic superficial gastritis→chronic atrophic gastritis→intestinal metaplasia→gastric dysplasia→gastric carcinoma. Combined detection of the two markers may help to study the mechanism of gastric cancer pathogenesis and development.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第23期2604-2609,共6页
World Chinese Journal of Digestology
基金
延安大学校内科研资助项目
No.YDK2006-29~~
