摘要
目的:观察新生大鼠缺氧缺血后脑内葡萄糖转运蛋白1(GLUT1)和葡萄糖转运蛋白3(GLUT3)的表达情况以及孕酮对其的影响。方法:新生SD大鼠40只,随机分成4组:正常组、假手术组、缺氧缺血组和孕酮组。建立新生鼠缺氧缺血性脑病模型,免疫组化方法检测新生大鼠海马部位GLUT1及GLUT3的表达。结果:正常组和假手术组新生大鼠海马可见少量GLUT1和GLUT3的表达,两组间无显著差异(P>0.05);缺氧缺血组GLUT1和GLUT3表达均明显高于假手术组(P<0.05);孕酮组GLUT的表达不仅明显高于假手术组(P<0.01),而且明显高于缺氧缺血组(P<0.05)。结论:孕酮通过上调GLUT1和GLUT3的表达以维持脑组织的能量供给,增强神经元对缺氧缺血的耐受性。
Aim： To investigate the expression of GLUT1 and GLUT3 in the hippocampus after cerebral hypoxic-ischemia（HI） in newborn rats and the effect of progesterone（PROG） on them. Methods： Forty newborn SD rats were randomly divided into four groups： normal group, sham-operated group, hypoxic-ischemic group and progesterone group. Model of hypoxic-ischemia encephalopathy（HIE） was established in the 7-day-old newborn SD rats. Immunohistochemical method was applied to detect the expression of GLUT1 and GLUT3 in hippocampus. Results： GLUT1 and GLUT3 were slightly seen in normal and sham operation group, there was no obviously difference between the two groups（ P〉0.05）. The expression of GLUT1 and GLUT3 in hypoxic-ischemia group were all higher than that in sham operated group（ P 〈 0.05）. Not only the expression of GLUT in progesterone group were significantly higher than that in sham operated group （ P 〈 0.01 ）, but also than that in hypoxic-ischemia group（ P 〈 0.05 ）. Conclusion： PROG could increase the tolerance of neuron to hypoxic-ischemia with maintaining the energy supply in the brain by up-regulating GLUT expression.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2008年第3期353-355,I0003,共4页
Chinese Journal of Applied Physiology
基金
河南省自然科学基金资助课题(984021100)
河南省医学科技创新人才工程项目(200024)
河南省教委科研项目(199310015)
