摘要
目的:探讨三种一氧化氮合酶抑制剂(NOSI)对盲肠结扎和穿孔法(CLP)败血症休克血流动力学改变及血管张力变化的影响。方法:采用CLP建立大鼠败血症休克模型,腹腔注射三种NOSI对CLP败血症休克进行干预;在体颈动脉插管和心室内导管术,测定血压和左心室动力学指标;用离体血管灌流方法,测定大鼠胸主动脉环的张力。结果:分别用三种NOSI可降低CLP败血症休克的死亡率,改善血压和左心室动力学指标;三种NOSI均能抑制CLP引起的去内皮主动脉环对苯肾上腺素(PE)和KCl的收缩反应性下降,并且iNOSI氨基胍(AMG)能够抑制CLP引起的去内皮主动脉环对细胞外钙的反应性降低;非选择性NOSI左旋硝基精氨酸甲酯(L-NAME)与nNOSI7-硝基吲唑(7-NI)均能抑制CLP引起的内皮完整主动脉环对苯肾上腺素(PE)和KCl的收缩反应性下降,但是三种NOSI均不能抑制CLP引起的内皮完整动脉环对细胞外钙的反应性降低。结论:三种NOSI均能改善CLP晚期败血症休克大鼠血流动力学和无内皮血管的收缩反应性,L-NAME和7-NI还可改善有内皮血管的收缩反应性。
Aim： To investigate the effect of three types of nitric oxide synthase inhibitors on the changes of hemodynamic parameters and thoracic aorta tension induced by septic shock in rats. Methods： We used cecal ligation and puncture（CLP） method to establish septic shock in rats, and the three types of nitric oxide synthase inhibitors were injected after CLP. The carotid artery was cannulated and connected to a pressure transducer to determine mean arterial blood pressure（MABP）. Ventricular dynamic parameters were determined following intraventricular cannulation via the carotid artery, including heart rate（HR）, left ventricular developed pressure （LVDP）, maximal rise/fall velocity of ventricular pressure（ ± dP/dtmax）. Isolated thoracic rings were mounted on an organ bath and the tension of the vessel was recorded. Results： ①After using L-NAME, AMG and 7-NI the mortality decreased to 50.0%, 37.5 % , and 42.1% , respectively （from 65.2 % in septic shock rats） ; ②The MABP in septic shock rats partly recovered after using the NOS inhibitors, all ventrlcular dynamic parameters partly recovered after using the inhibitors; ③The hyporeactivity of endotheliurn-denuded aortic rings to vasoconstrictors induced by septic shock was partly recovered by pretreatment with the inhibitors. However, only L-NAME or 7-NI could inhibit the decrease of vasoconstriction induced by septic shock in endothelium-intact aortic rings. Conclusion： The three types of nitric oxide synthase inhibitors can improve the hemodynamic parameters and vasoconstriction responsiveness of endothelium-denuded aorta of septic shock rats. Furthermore, L-NAME and 7-NI improve the responsiveness of endothelium-intact aorta.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2008年第3期279-284,共6页
Chinese Journal of Applied Physiology
基金
浙江省嘉兴市科技局科研项目资助(2005AY3044)
关键词
败血症
左心室动力学
胸主动脉
一氧化氮合酶
一氧化氮合酶抑制剂
septic shock
ventricular-dynamie parameters
thoracic aorta rings
nitric oxide synthase
nitric oxide synthase inhibitor
