摘要
目的:探讨斑点杂交法检测结核分枝杆菌的临床应用价值。方法:用抗酸染色法、L-J培养法、PCR法和斑点杂交法平行检测76例临床标本和28种标准菌株。结果:46例肺结核患者痰标本抗酸染色法、L-J培养法、PCR法和斑点杂交法结核分枝杆菌检测阳性率分别为43.4%、50.0%、73.9%和56.5%;30例非结核患者痰标本、19种非结核分枝杆菌和9种非分枝杆菌标准株培养法和斑点杂交法检测结果均为阴性,PCR法有3例阳性。结论:斑点杂交法检测结核分枝杆菌方法简便,结果可靠,可用于结核病的快速诊断。
Objective： To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. Methods： Acid fast stain , L- J culture ,PCR and dot blot hybridization was uesd to detect the seventy - six clinical isolates and twenty - eight kinds of normal tuberculosis. Result： Acid fast stain L - J culture ,PCR ,dot blot hybridization was used to detect the mycobacterium tuberculosis of sputum of forty - six pulmonary tuberculosis,and their positive rate was 43.4% ,50.0% ,73.9% ,56.5% respectively. The re- sults of thirty non - pulmonary tuberculosis , nineteen kinds of non - mycobacterium tuberculosis and nine kinds of non - mycobacteri which was detected by culture and dot blot hybridization were all negative. The results of three specimens were positive when they were detected by PCR. Conclusion：Using dot blot hybrid- ization to detect mycobacterium tuberculosis is simplified and the results is reliable. It can be uesd to rapid diagnosis of TB.
出处
《河北医学》
CAS
2008年第10期1211-1213,共3页
Hebei Medicine
关键词
结核分枝杆菌
斑点杂交
抗酸染色
L-J培养
Mycobacterium tuberculosis
Dot blot hybridization
Acid fast stain
L- J culture
