摘要
目的了解大鼠神经元对1．8GHz移动电话射频电磁场（RFEMF）的反应基因，及在不同辐照时间、不同辐照模式下的基因表达。方法抽提经1．8GHz，SAR为2W／kg的射频电磁场辐照和假辐照24h后原代培养神经元的RNA，用基因芯片筛选其差异表达基因，反转录-聚合酶链反应（RT-PCR）验证某些基因（Egr-1、Mbp和PIp），并观察在不同辐照时间（6、24h）、不同辐照模式（间断辐射与连续辐射）下的表达情况。结果在1200个候选基因中，24个上调，10个下调，差异表达基因与多种细胞功能相关（细胞骨架、信号通路、细胞代谢等）。RT—PCR验证Egr-1、Mbp和PIp基因的变化与芯片结果较一致。神经元暴露于1．8GHz2W／kg射频间断辐射24、6h后，Egr-1、PIp基因表达发生明显改变，差异有统计学意义LP〈0．05），而Mbp的改变无统计学意义（P〉0．05）；暴露于24h连续辐射后，Egr-1、Mbp基因表达发生明显的改变，差异有统计学意义（P〈0．05），而PIp的改变无统计学意义（P〉0．05）；暴露于6h连续辐射后，Egr-1、Mbp、PIp基因表达均未发生明显的改变。不同辐照时间（24、6h相比）和不同辐照模式（间断辐射和连续辐射相比）对神经元Egr-1、Mbp、PIp基因表达的影响存在明显差异。结论1．8GHz射频辐射影响大鼠神经元多个基因的表达；Egr-1基因表达下调、Mbp和PIp基因表达上调，提示其对神经元可能产生不良影响；1．8GHz射频间断辐射引起大鼠神经元细胞基因表达改变的效应大于连续辐射；24h射频辐照（间断或连续）引起大鼠神经元细胞基因表达改变的效应大于6h射频辐照（间断或连续）。
Objective To investigate the changes of gene expression in rat neuron induced by 1.8 GHz radiofrequency electromagnetic fields （RF EMF） to screen for RF EMF-responsive genes and the effect of different exposure times and modes on the gene expression in neuron. Methods Total RNA was extracted immediately and purified from the primary culture of neurons after intermittent exposed or sham-exposed to a frequency of 1.8 GHz RF EMF for 24 hours at an average special absorption rate （SAR） of 2 W/kg. Affymetrix Rat Neurobiology U34 array was applied to investigate the changes of gene expression in rat neuron. Differentially expressed genes （Egr-1, Mbp and Pip） were further confirmed by semi-quantitative revere transcription polymerase chain reaction （RT PCR）. The expression levels of Egr-1, Mbp and Pip were observed at different exposure times （6, 24 h） and modes （intermittent and continuous exposure）. Results Among 1200 candidate genes, 24 up-regulated and 10 down-regulated genes were found by using Affymetrix microarray suite software 5.0 which are associated with multiple cellular functions （cytoskeleton, signal transduction pathway, metabolism, etc.） after functional classification. Under 24 h and 6 h intermittent exposure, Egr-1 and Pip in experiment groups showed statistic significance （P〈0.05） compared with the control groups, while expression of Mbp did not change significantly （P 〉 0.05 ）.After 24 h continuous exposure, Egr-1 and Mbp in experiment groups showed statistic significance （P〈0.05） compared with the control group, while expression of Pip did not change significantly（ P 〉 0.05 ）. Under the same exposure mode 6 h, expression of all the 3 genes did not change significantly. Different times （6, 24 h） and modes （intermittent and continuous exposure） of exposure exerted remarkable different influences on the expression of Egr-1 ,Mbp,Plp genes （P〈0.01）. Conclusion The changes of many genes transcription were involved in the effect of 1.8 GHz R
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2008年第8期449-452,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
辐射
神经元
基因表达
Radiation
Neurons
Gene expression
