摘要
目的观察兔肝癌行TAE和TACE治疗后HIF-1α,MVD,PCNA的变化情况,探讨其治疗机制。方法30只新西兰大白兔VX2瘤细胞株种植瘤成模后1周分别给予肝动脉注入生理盐水(对照组)和TAE及TACE治疗。治疗2周后观察肿瘤大小,肿瘤中HIF-1α,MVD,PCNA的变化。结果3组动物的肝左叶均见肿瘤生长,对照组、TAE组和TACE组分别见15,11,3个新生结节。对照组HIF-1α和PCNA较TAE组明显减弱(P<0.05);但较TACE组中明显增强,与其他两组比较差异均有显著性(P<0.05);TAE组肝肿瘤MVD[(115.3±20.74)/mm2]明显高于对照组[(72.11±18.95)/mm2]和TACE组MVD[(47.33±15.53)/mm2],差异有显著性(P<0.01),TACE组MVD明显低于对照组和TAE组(P<0.05)。结论TAE术后肿瘤组织缺血缺氧加重,HIF-1α和PCNA蛋白表达增加,可能促进肿瘤细胞增生;TACE术后肿瘤组织中HIF-1α和PCNA蛋白表达减少,有抑制肿瘤细胞增生的作用。
Objective To investigate expression and clinical significance of HIF—1α, MVD and PCNA after TAE or TACE for rabbit liver carcinoma, and to identify the mechanism of hepatic carcinoma cell proliferation after the operation. Methods Thirty rabbits were implanted with liver VX2 tumors for 1 week. Then they were randomly divided into 3 groups. Control group had hepatic arterial infusion of 0.5 to 0.8 mL saline. TAE group was embolized with O. 5 to 0. 8 ml UFLP. TACE group was embolized with 0. 5 to 0. 8 ml UFLP mixed with 2 mg THP. Two weeks after treatment, HIF—1α, MVD and PCNA expression was detected. Results Growth of the liver tumor was found in the 3 groups, and there were 15 , 11 and 3 new tumors found in the control group, TAE group and TACE group respectively. The expressions of HIF—1αand PCNA in control group were higher than those in TACE group, but significantly lower than those in TAE group （ P 〈 0.05）. The MVD in TAE group（115.3 ±20.74/ mm^2） was higher than that in control group （72.11± 18.95 / mm^2 ） and TACE group （ 47.33 ± 15.53 / mm^2 ） （ P 〈 0.05 ）. Conclusions The expressions of HIF-1α and PCNA were higher after TAE and can promote tumor growth. The expressions of HIF-1 α and PCNA were lower after the TACE and can suppress tumor growth.
出处
《中国普通外科杂志》
CAS
CSCD
2008年第8期777-780,共4页
China Journal of General Surgery
关键词
肝肿瘤/治疗
化学栓塞
治疗性
缺氧诱导因子
细胞增殖核抗原
Liver Neoplasms/ther
Chemoembolization, Therapeutic
Hypoxia Inducible Factor
ProliferatingCell Nuclear Antigen
