摘要
来自同一祖先的两株不同亚克隆细胞系Ｈｃａ－１６Ａ３－Ｆ（Ｆ）和ＨＣａ－Ａ２－Ｐ（Ｐ），在两种不同小鼠体内经不同途径接种所得的转移率和转移部位显示有差别：Ｃ３／ｈｅ小鼠皮下组，Ｆ呈现高淋巴道转移（８０．０％），而Ｐ未出现转移，Ｃ３Ｈ／ｈｅ小鼠尾静脉组，Ｆ在卵巢（２８．６％）和淋巴结（２８．６％）有较低的转移率，Ｐ权出现一例（１４．３％）卵巢转移，然而，在裸鼠体内却出现了异常的变化，Ｆ呈现高血道（肺脏７１．４％）转移而不出现淋巴道和其他部位转移，但Ｐ却出现高淋巴道转移（８５．７％）兼有低血道（肺脏２８．６％，卵巢１４．３％，肾脏２８．６％）转移。体内实验结果提示：ＨＣａ细胞中含有转移、浸润能力不同的亚群；Ｆ细胞在体内具有较强的浸润力与转移力，且有淋巴道转移的倾向；Ｐ细胞的浸润力和转移力则低于Ｆ细胞，但若给其提供适当的到达器官的通路，完全能形成实验性转移灶。Ｃ３Ｈ／ｈｅ小鼠也是ＨＣａ细胞生长和转移的理想宿主。
HCa-F and HCa-P cell lines are two clones isolated from HCa cell line.After intravenous and subcutaneous tumor-cell inoculation,the different sites and rate of metastasis by HCa-F and HCa-P cell were seen in two recipient mice.HCa-F resulted in highly lymphatic metastasis(80%) in C3H/hej mice with subcutaneous injection in contrast, HCa-P was free from metastasis;in C3H/hej mice with intravenous inoculation,HCa-F cell produced 28.6% colonies in ovary and 28.6% colonies in LN, HCa-P cell cnly showed 14.3% metastasis in ovary.In athymic mice inoculated from tail vein,unusual experimental metastsis appeared.HCa-F cell formed high lung metastasis(71.4%) not accompanied by other organs involved,recipient suffered extensive metastasis by HCa-P(LN 85.7%;lung 28.6%;ovary 14.3%; hidney 28.6%).The results from in vivo experiments suggest that thare are different with difffering the capacity of invasion and metastasis.in HCa cells;HCa-F cell line show higher potential of invasion and metastasis in vivo and affinity to lymphatic system;HCa-P cell line is a cell line with much lower invasive and metastatic ability than HCa-F cell line,but they can form experimental metastasis if provided with vascular access to an organ;C3H/hej mice can be host for cells to be transplanted and metastasiszed.
基金
国家自然科学基金
美国NCI资助
关键词
肝癌
亚克隆细胞系
hepatocarcinoma
subclonal cell line
