摘要
通过对钼锑抗比色法测定有效磷含量测定值影响因素的对比分析,以便获取快捷准确地评价磷细菌的解磷能力的方法,进行有效地筛选。实验证明反应液最大吸收波长在720 nm左右;发酵体积、加入活性碳与否、反应液的pH值等对测定结果影响不大,这样在筛选时就可以大大提高效率;超声波处理20 min效果明显;在用50 mL容量瓶进行定容时,取样体积以2.5 mL为宜。对发酵液进行离心效果更好些,但在必要的情况下,可以静置后取上清测定。
Preliminary study on factors affecting the determined value by Mo - Sn - Vc method of organic phosphobacteria was made. The maximum absorption wavelength was about 720nm when mo- sb - vc - method was employed to determine the effective phosphorus of the fermentation broth. The fermentation volume, adding of active carbon and pH value of reaction solution had no significant effect on the resuit, and this will help improve screening efficiency. The ultrasonic treatment for 20 min had notable effects on the results. And it was also found that 2. 5 mL could be considered the appropriate sampling volume when 50 mL volumetric flask was used. The centrifugation of fermentation broth could lead to comparatively better results, nevertheless the supernatant after standing could be sampled when necessary.
出处
《湖州职业技术学院学报》
2008年第3期1-3,共3页
Journal of Huzhou Vocational and Technological College
基金
长江大学引进博士启动基金资助项目<离子束细胞修饰技术在磷钾细菌改良中的应用>(00324)
关键词
菌种选育
解磷微生物
磷细菌
钼锑抗法
strain breeding
phosphorus - dissolving microbe
phosphobacteria
mo- sb - vc - method
