摘要
目的：建立化学发光法测定左炔诺孕酮的含量。方法：采用YN—FG化学发光仪，以硫酸铈-亚硫酸钠为发光体系测定左炔诺孕酮的含量。结果：左炔诺孕酮在0．025～0．4g·mL^-1范围内，进样量与峰面积呈良好线性关系，r=0．9961，其日内RSD小于6.08％，日间RSD小于13．24％，该方法的检出限为0．0025g·mL^-1。结论：该方法灵敏度高，线性范围宽，简单快速，可用于左炔诺孕酮含量的测定。
Objective：To establish a chemiluminescence method for the concentration determination of levonorgestrel. Methods： It was based on the reaction of sodium sulfite with cereus sulfate in the phosphonic acid solution. Results： The chemiluminescence intensity was proportional to levonorgestrel concentration over the range of 0. 025 - 0.4 g· mL^ - 1. The detection limit was 0. 002 5 g· mL^ - 1. The relative standard deviation in intra-day and inter-day was less than 6.08%, 10.88%, respectively. Conclusion： This method can be successfully applied to the determination of levonorgestrel.
出处
《药学与临床研究》
2008年第4期322-324,共3页
Pharmaceutical and Clinical Research
关键词
化学发光
硫酸铈-亚硫酸钠体系
左炔诺孕酮
Chemiluminescence
Cerous sulfate-sodium sulfite system
Levonorgestrel
