摘要
目的从肿瘤坏死因子-α(TNF-α)、粘附分子(ICAM-1、VCAM-1)表达变化揭示补阳还五汤抗老年脑缺血再灌注损伤的机制。方法采用大脑中动脉栓塞(MCAO)方法复制脑缺血动物模型,观察缺血(I)3h和缺血再灌注(I/R)1,3,6,12d神经症状积分、脑组织含水量、病理变化、TNF-α、VCAM-1、ICAM-1和ICAM-1mRNA表达变化。结果模型组脑组织含水量(I/R1,3,6d)、神经症状积分(各时间点)和TNF-α、VCAM-1(I3h、I/R1,3,6d)、ICAM-1(I3h、I/R1,3,6d)及其mRNA(各时间点)均高于假手术组;补阳还五汤组神经症状积分、脑组织含水量(I/R3,6,12d)、TNF-α、VCAM-1和ICAM-1及其mRNA(I3h、I/R1,3d)表达均低于模型组;补阳还五汤组神经症状积分(I/R6,12d)、TNF-α与ICAM-1(I/R1d)及其mRNA(I/R1,3d)表达低于尼莫地平组。结论补阳还五汤抗老年脑缺血再灌注损伤的机制与其抑制TNF-α、VCAM-1、ICAM-1等表达有关。
Objective To find out the protective mechanism of Buyanghuanwu Decoction for antagonizing senile cerebral ischemia-reperfusion lesion on the base of expression changes of tumor necrosis factor-α （TNF-α）,intercellular cell adhesion molecule-1 （ICAM-1） and vascular cell adhesion molecule-1 （VCAM-1）. Method The animal model of cerebral ischemia was established by using middle cerebral artery occlusion （MCAO）. The nervous symptom scores,brain water content and pathological changes,and the expressions of TNF-α,ICAM-1,VCAM-1 and ICAM-1mRNA were observed after ischemia for 3 hours,and ischemia-reperfusion lesion for 1,3,6 and 12 days respectively. Result In the model group the brain water content （ischemia-reperfusion for 1,3 and 6 days）,nervous symptom scores （at all time points）,expressions of TNF-α and VCAM-1 （ischemia for 3 hours,ischemia-reperfusion for 1,3 and 6 days）,ICAM-1 （ischemia for 3 hours,ischemia-reperfusion for 1,3 and 6 days） and mRNA （at all time points） were all higher than those in the sham-operation group. In the Buyanghuanwu Decoction group the nervous symptom scores and brain water content （ischemia-reperfusion for 3,6 and 12 days）,the expressions of TNF-α,VCAM-1,ICAM-1 and mRNA （ischemia for 3 hours,ischemia-reperfusion for 1 and 3 days） were all lower than those in the model group. In the Buyanghuanwu Decoction group the nervous symptom scores （ischemia-reperfusion for 6 and 12 days）,the expressions of TNF-α （ischemia-reperfusion for 1 day）,ICAM-1 （ischemia-reperfusion for 1 day） and mRNA （ischemia-reperfusion for 1 and 3 days） were all lower than those in the nimodipine group. Conclusion The mechanism of Buyanghuanwu Decoction for antagonizing senile cerebral ischemia-reperfusion lesion may be related to its inhibitory effect on the expressions of TNF-α,ICAM-1 and VCAM-1.
出处
《北京中医药大学学报（中医临床版）》
2008年第4期1-4,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.30371812)
关键词
补阳还五汤
老年
脑缺血再灌注
肿瘤坏死因子
粘附分子
Buyanghuanwu Decoction
senile
cerebral ischemia-reperfusion
tumor necrosis factors
cell adhesion molecules
