摘要
目的:观察胰岛素强化治疗对危重症并高血糖患者外周血单核细胞(PBMC)核转录因子κB(NF-κB)活性及其基因表达的影响。方法:120例高血糖危重症患者随机分为胰岛素强化治疗组(强化组)和胰岛素常规治疗组(常规组)。于入院时、入院后第1d、入院后第2d取血2mL,提取外周血单核细胞(PBMC),酶联免疫吸附法(ELISA)检测PBMC中NF-κB活性,RT-PCR检测NF-κBmRNA的表达情况;30例健康志愿者作为对照组。结果:强化组和常规组在所有时间点NF-κB活性和NF-κBmRNA表达均显著高于对照组(P<0.05);但强化组入院后第1d、第2dNF-κB活性和NF-κBmRNA表达则低于常规组(P<0.05)。结论:胰岛素强化治疗能够显著降低危重症合并高血糖患者外周血单核细胞NF-κB的活性及其mRNA的表达,可能是其降低患者死亡率的重要原因。
objective To investigate the effects of intensive insulin therapy on the activity and gene expression of nuclear factor-κB （NF-κB） in peripheral blood mononuclear cells（PBMC） in patients with critical illness complicated by hyperglycemia. Methods One hundred and twenty patients with critical illness complicated by hyperglycemia were randomly divided into intensive group （ received intensive insulin therapy and maintenance of blood glucose at a level between 4.4 - 6.1 mmol/L, n = 60）and conventional group（ received conventional treatment and maintenance of blood glucose at a level between 8.0 - 11.1 mmol/L, n = 60） , in addition,30 healthy volunteers were used as control group （ n = 30 ）. The activities of NF-κB in PBMC were assessed with ELISA, and NF-κB mRNA expression was assessed with RT-PCR. Results Compared with control group ,the activities and mRNA expression of NF-κB in PBMC were significantly decreased in intensive group and conventional group at all the time points, but in intensive group the activities and mRNA expression of NF-κB in PBMC were lower than conventional group at the 1 st and 2nd day after admission. Conclusion Intensive insulin therapy can significantly inhibit the activity and gene expression of nuclear factor-κB in peripheral blood mononuclear cells in patients with critical illness complicated by hyperglycemia, which is probably an important mechanism to reduce mortality of these patients.
出处
《郧阳医学院学报》
2008年第4期328-330,333,共4页
Journal of Yunyang Medical College
