摘要
目的:探讨新辅助化疗前后P-糖蛋白(P-glycoprotein,P-pg)和多药耐药相关蛋白1(Multiple resistance-associated protein-1,MRP1)在口腔鳞癌组织中的表达及其与化疗敏感性和相关临床病理因素的关系。方法:采用SP免疫组化法,对106例口腔鳞癌患者新辅助化疗前后P-pg和MRP1的表达水平进行检测,分析其表达水平与化疗疗效的关系。结果:(1)化疗后口腔鳞癌组织中P-pg阳性表达率为74.53%,显著高于化疗前41.51%(P﹤0.05);化疗前P-pg表达阴性患者有效率为56.45%,显著高于P-pg表达阳性患者的有效率34.09%(P﹤0.05);P-pg的表达水平与口腔鳞癌的分化程度呈正相关(P﹤0.05),但与临床分期和是否有颈淋巴结转移不相关(P﹥0.05);(2)MRP1在口腔鳞癌组织中的阳性表达率在化疗前后分别为49.06%、57.55%,两者间无显著性差异(P﹥0.05);化疗前MRP1表达阴性患者和阳性患者的有效率分别为50.00%、44.23%,两者间无显著性差异(P﹥0.05);MRP1的表达水平与口腔鳞癌的分化程度、临床分期和颈淋巴结转移均不相关(P﹥0.05)。结论:P-pg和MRP1在口腔鳞癌组织中均有较高的表达水平,但只有P-pg的表达水平与新辅助化疗疗效具有显著的相关性,认为P-pg的表达水平可作为预测口腔鳞癌对化疗敏感性的分子标记物。
Objective：To investigate the expression of Multiple resistance-associated protein-1（MRP1） and P-glycoprotein（P-pg） in oral squamous cell carcinoma（OSCC） before and after neoadjuvant chemotberapy（NACT） and its relation with tumor chemosensitivity and related clinical pathological factors. Methods：The specimens of 106 patients with OSCC hefnre and after NACT were examined by SP immunnhistochemistry. The correlation between P-pg and MRP1 expression and therapeutic effect was analyzed. Results：（1）P-pg positive expression rate in OSCC after NACT was 74.53%,which was significantly higher than thatbefore NACT （41.51%）（P 〈 0.05）;the effective rate of patient with P-pg negative expression before chemotherapy was 56.45%,which was significantly higher than that of patient with P-pg positive expression（34.09%）（P 〈 0.05）;P-pg expression levels were positively relative to the differentiation of the tumor（P 〈 0.05）,but not relative to clinical stages and cervical lymph node metastasis（P 〉 0.05）; （2）MRP1 positive expression rates in OSCC before and after NACT were 49.06%,and 57.55% respectively and the difference was not significant（P〉 0.05）;the effective rates of patient with MRP1 negative expression in OSCC before chemotherapy were 50.00% and 44.23% respectively and the difference was not significant（P〉 0.05）;MRP1 expression levels were not relative to clinical stages, differentiation of the tumor or cervical lymph node metastasis（P〉 0.05）; Conclusion：P-pg and MRP1 have high expression in primary oral squamous cell carcinoma, hut only P-pg expression levels are significantly associated with response to chemotherapy and it can be used as a predictive marker of the sensitivity to chemotherapy in patients with OSCC.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第8期931-933,1015,共4页
Journal of Chongqing Medical University
基金
重庆市科委应用基础研究项目(2003-43)
重庆市卫生局科研项目(2-073)
关键词
新辅助化疗
多药耐药相关蛋白
P-糖蛋白
口腔鳞癌
Neoadjuvant chemotherapy
Multiple resistance-associated protein （MRP）
P-glycoprotein （P-pg）
Oral squamous cell carcinoma
