摘要
目的研究葡萄糖激酶相关双特异性磷酸酶12基因（DUSP12）-6735位点多态性与中国人2型糖尿病的关系。方法选取577名上海地区中国人（其中糖耐量正常者359名，新诊断2型糖尿病患者218例），用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）检测DUSP12基因-6735T→C变异，并检测所选人群糖脂代谢，体脂分布等临床指标。结果糖尿病组与正常糖耐量组间DUSP12基因-6735T→C多态位点各基因频率分布差异无统计学意义（P〉0．05）。在正常糖耐量者中，此多态c等位基因携带者空腹血糖水平高于Tr基因型者[5．00（4．69～5．28）mmol／L vs 4．90（4．50～5．26）mmol／L，P=0．035]，并且C等位基因携带者血清高密度脂蛋白胆固醇低于TT基因型者[（1．25±0．30）mmol／L vs （1．34±0．30）mmol／L，P=0．010]。结论DUSP12基因-6735T→C变异与2型糖尿病无直接相关，但是与糖脂代谢有一定相关性。
Objective To investigate the impact of glueokinase-assoeiated dual-specificity phosphatase 12 gene （DUSP12） single nucleotide polymorphism （SNP） on type 2 diabetes mellitus in Chinese. Methods The genotypes of -6735T→C of DUSP12 were determined by PCR-RFLP in 577 Chinese in Shanghai, 359 with normal glucose tolerance （NGT） and 218 being newly diagnosed DM patients without taking any drug. Oral glucose tolerance test was conducted. Height, weight, glucose and insulin concentrations, serum lipid profiles, and fat distribution parameters were determined. Results The genotype frequency distributions of -6735T→C variant in DUSP12 gene of the DM group and NGT group were not significantly different （ all P 〉 0.05 ）. The fasting plasma glucose concentration of the NGT subjects with C allele was 5.00 （4.69 - 5.28 ） mmol/L, significantly higher than that of the subjects with TT genotype [ 4. 90 （4.50 - 5.26） mmol/L, P = 0. 035 ] . The serum HDL-c concentration of the NGT subjects with C allele was （ 1.25 ± 0. 30） mmol/L, significantly lower than that of those with TT genotype [ （ 1.34 ± 0. 30） mmol/L, P = 0.010]. These two results still showed significant statistical differences after adjusted with sex, age, and BMI （P = 0. 035）. Condusion -6735T→C variant in DUSP12 doesn＇t play a major role in diabetes, but it may have some effects on glucose and lipid metabolism.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第32期2250-2253,共4页
National Medical Journal of China
基金
国家自然科学基金资助项目（C030301）
上海市医学领军人才培养计划基金资助项目（LJ06010）
