摘要
目的观察苦参素(Oxymatrine,OMT)对感染性休克大鼠肺损伤的防治作用。方法采用大鼠盲肠结扎穿孔法(cecal ligation and puncture,CLP)复制大鼠感染性休克模型,将56只大鼠随机分为假手术组、OMT对照组、模型(CLP)组、CLP+OMT 52、26、13 mg/kg剂量组、阳性对照(CLP+地塞米松)组。通过观察血气、肺组织W/D比值、肺系数及肺含水量、肺病理学检查(大体改变及肺病理组织切片)等指标,对肺损伤进行评估。结果CLP组较假手术组比较PaO2、SaO2及BE值均降低(P<0.01);PaCO2、HCO3、W/D比值、肺系数及肺含水量均升高(P<0.05)。CLP+OMT 522、6、13 mg/kg剂量组及CLP+地塞米松组较CLP组比较PaO2、SaO2及BE值均升高;PaCO2、HCO3、W/D比值、PWI及肺含水量均降低(P<0.01或P<0.05);CLP+OMT 52、26、13 mg/kg剂量组及大鼠双肺肉眼观察与CLP组相比,体积膨大程度有所减轻,淤血较轻,颜色较浅。结论OMT 13、26、52mg/kg对感染性休克大鼠肺损伤性病变有明显的抑制作用。
Objective To explore the preventive and therapeutic effects of oxymatrine（OMT）on lung injury in the rat model of septic shock.Methods Fifty-six male SD rats were randomly divided into 7 groups：sham operation group,OMT control group,model（CLP）group,CLP＋OMT high,middle-dose group,low-dose group,positive control group.The pulmonary function was evaluated with blood gas,the ratio between wet weight of the lung and dry weight of the lung,PWI and pulmonary moisture capacity,gross changes and pathological changes examined with lightmicroscope in the pulmonary tissue.Results OMT could significantly elevate the content of PaO2,SaO2,significantly decrease the content of PaCO2,HCO-3（P〈0.05）,significantly decrease the ratio between wet weight of the lung and dry weight of the lung,PWI and pulmonary moisture capacity（P〈0.05）,improve the condition of pulmonary hyperemia、edema,infiltrate of heterophil granulocyte and emerge of asphyxial membrane and alleviate the inflammatory reaction.Conclusion OMT could antagonize the lung injury in a rat model of septic shock.
出处
《宁夏医学院学报》
2008年第4期421-423,F0003,共4页
Journal of Ningxia Medical College
基金
宁夏医学院2007年面上项目(XM200712)
