摘要
建立了鸡肉组织中甲硝唑、洛达硝唑、地美硝唑、替硝唑、塞克硝唑、奥硝唑6种硝基咪唑类药物及地美硝唑代谢产物1-甲基-5-硝基-2-羟甲基咪唑（HMMNI）共7种组分残留检测的高效液相色谱法（HPLC）。样品采用乙酸乙酯提取，SCX柱净化；色谱条件：色谱柱为Eclipse Plus C18柱（4．6mm×150mm，3．5μm）；流动相为水-乙腈-甲醇，梯度洗脱；柱温为35℃；流速为0．8mL／min，紫外检测波长为320nm，外标法定量。结果表明：7种组分在5～60ng／mL范围内，呈良好线性关系，相关系数R^2均大于0．998；方法检出限为1μg／kg；添加浓度分别为2、3、5μg／kg时，平均回收率为66．6％～101％，批内和批间RSD均小于20％。
An HPLC method was established for determination of seven nitroimidazole residues in chicken, including metronidazole, ronidazole, tinidazole, secnidazole, ornidazole, dimetridazole and 2 - hydroxymethyl - 1 - methyl- 5 -nitroimidazole （HMMNI）. The sample was extracted with ethyl acetate, and then was purified by strong cation exchange（SCX） solid phase extraction cartridge; HPLC conditions： the chromatography column was Eclipse Plus C18 column of 4.6 mm × 150 mm, 3.5 μm; mobile phase was water - acetonitrile - methanol; column temperature was 35 ℃ ; flow rate was 0.8 mL/min; detection, wavelength was 320 nm. The calibration curve was good linear between the peak areas and concentrations of nitroimidazole from 5 to 60 ng/mL with the correlation coefficient R^2 〉 0. 998. The limit of detection was 1 μg/kg. The average recoveries from spiked chicken at the three concentrations of 2, 3 and 5 μg/kg ranged from 66.6% -101%, and intra- day and inter- day （n = 5 ） batch variation coefficients were less than 20%.
出处
《中国兽药杂志》
2008年第8期9-12,共4页
Chinese Journal of Veterinary Drug
