摘要
目的探讨环氧化酶（COX）-2基因启动子区的-1290A〉G，-1195G〉A和-765G〉C单核苷酸多态与胰腺癌发生风险的关系。方法对象包括283例胰腺癌患者和566名正常对照组。采用PCR．限制性片段长度多态方法进行COX-2基因启动子区-1290A〉G，-1195G〉A和-765G〉C多态的基因型分析，不同基因型与单体型携带者发生胰腺癌相对风险度的评估使用比值比（OR）及95％可信区间（CI）。结果多变量Logistic回归分析显示-1195AA和-765GC基因型与胰腺癌发生的风险升高相关，OR值分别为1．75（95％CI=1．16～2．64）和2．53（95％CI=1．43～4．47）。单体型分析显示：与A-1290-G-1195-G-765相比较，含有-1195A等位基因的A—1290-A-1195-G-765和G-1290-A-1195-C-765，两种单体型携带者发生胰腺癌的相对风险升高，OR值分别为1．26（95％CI=1．02～1．56）和5．54（95％CI=1．79～17．16）。-765CG和-1195AA基因型与吸烟具有交互作用，共同增加胰腺癌发生的风险。结论COX-2基因启动子区的-1195G〉A和-765G〉C单核苷酸多态与胰腺癌遗传易感性相关。
Objective To evaluate the effects of - 1290A 〉 G, - 1195G 〉 A and - 765G 〉 C single nucleotide polymorphisms （SNPs） in the promoter of cyclooxygenase （COX）-2 gene on the risk of pathogenesls of pancreatic cancer. Methods Peripheral blood samples were collected from 283 patients with pancreatic cancer and 566 normal controls. Questionnaire survey was conducted to understand the demographic data and status of smoking and smoking cessation of the subjects. Polymerase chain reaction- based restriction fragment length polymorphism （PCR-RFLP） was used to detect the genotypes of the gene fragments containing the 3 SNP sites in the promoter regions of the COX-2 gene. Statistical tests were performed to analyze the relations among different factors and the risk of pancreatic cancer. Results Three SNPs, -1290A 〉 G, - 1195G 〉 A, and -765G 〉 C were identified. A case-control analysis revealed 1.75-fold （95% C1 = 1. 16 - 2.64） and 2. 53-fold （95% CI = 1.43 - 4.47） excesses of risks of developing pancreatic cancer for the - 1195AA and - 765CG genotype carriers respectively compared with the non-carriers. Compared with A 1290-G-1195-G-765 containing haplotype, greater risks of developing pancreatic cancer were observed for A_1290-A_1195-G_765 （ OR = 1.26,95 % , CI = 1.02 - 1.56 ） and G_1290- A_1195-C_765 （ OR =5.54,95% CI = 1.79 - 17.16） containing haplotypes. There were interactions between the -765CG or -1195AA genotype and smoking in the risk of developing pancreatic cancer. Conclusion The SNP of - 1195A 〉 G and -765G 〉 C in the COX -2 promoter may play an important role in mediating hereditary susceptibility to developing pancreatic cancer.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第28期1961-1965,共5页
National Medical Journal of China
关键词
胰腺肿瘤
环氧化酶2
多态性
单核苷酸
Pancreatic neoplasms
Cyclooxygenase 2
Polymorphlsm,single nucleotide
