摘要
目的:探讨麦麸多肽的毒性作用。方法:应用急性毒性试验、Ames试验、骨髓细胞微核试验、睾丸精母细胞染色体畸变试验、彗星试验对麦麸多肽进行毒性研究。结果:麦麸多肽灌胃,对小鼠LD50>20g/kg,属无毒级。Ames试验中TA97、TA98、TA100和TA102的4个标准菌株无论代谢活化与否,皆为阴性结果;骨髓细胞微核试验、睾丸精母细胞染色体畸变试验、彗星试验2.5、5.0、10.0g/kg bw3个剂量组与阴性对照组比较差别无统计学意义。因此,Ames试验、骨髓细胞微核试验、睾丸精母细胞染色体畸变试验、彗星试验结果均为阴性。结论:麦麸多肽是一种无毒物质。
Objective： To study the toxicity of wheat bran peptide. Methods： The Ames test, bone marrow polychromatic erythrocyte micronucleus test, spermatogonial chromosomal aberrations test and comet assay were used to detect the toxicity of wheat bran peptide. Results： The LD50 of wheat bran peptide by i.g. to mice is more than 20 g/kg, no toxicity existing. If S9 activation or not, there are all negative results of the TA97, TA98, TA100 and TA102 strains in Ames test. In bone marrow polychromatic erythrocyte micronucleus test, spermatogonial chromosomal aberrations test and comet assay, there are not significant differences between the negative control and the treated groups （p 〉0.05） in dose of 2.5, 5.0, 10.0 g/kg bw respectively. The results of genetic toxicity tests are all negative in Ames test, bone marrow polychromatic erythrocyte micronucleus test, spermatogonial chromosomal aberrations test and comet assay. Conclusion： Wheat bran peptide has no toxicity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第8期569-572,共4页
Food Science
基金
辽宁省科技厅科技攻关项目(2004205001)
关键词
麦麸多肽
AMES试验
微核试验
睾丸精母细胞染色体畸变试验
彗星试验
wheat bran peptide
Ames test
bone marrow polychromatic erythrocyte micronucleus test
spermatogonial chromosomal aberrations test
comet assay
