摘要
目的研究红景天苷(salidroside)对水通道蛋白4(aquaporin 4,AQP4)在大鼠缺血性脑组织中表达的影响。方法采用线拴法制作大鼠局灶性脑缺血模型,实验分为模型组、假手术组、红景天苷组。通过光镜技术观察各组别脑水肿区的病理变化,干湿质量法检测脑含水量,采用原位杂交、免疫组化方法观察AQP4及其mRNA在脑组织中的表达,硫代巴比妥酸法和定磷法测定缺血脑组织中丙二醛(MDA)、Na+,K+-ATPase活性。结果缺血后模型组的细胞及血管周围间隙明显扩大,部分细胞坏死,神经胶质细胞增生;大鼠穹隆下器官、脉络丛、大脑皮质、视上核、海马等部位AQP4及其mRNA的表达上调,48～72 h达峰值;脑组织含水量亦在72 h达高峰。红景天苷组脑组织形态结构无明显改变,脑组织含水量、MDA水平、AQP4及其mRNA的表达明显低于模型组;而Na+,K+-ATPase活力明显高于模型组。结论红景天苷可抑制缺血性脑水肿组织中AQP4及其mRNA的过表达,防止脑水肿的发生。
Objective To study the ischemic cerebral tissues of rats. Methods effect of salidroside on aquaporin 4 （AQP4） expression in the A total of 160 healthy adult Wistar rats were divided into three groups randomly, the model group, the Sham group, and the salidroside group. MCAO Models were established by occluding unilateral middle cerebral artery （MCA） of the rats with the suture method. The morphological changes were observed with HE stain. Brain water content （BWC） was measured by using wet-dry weighing method. The expression of AQP4 and its mRNA in the cerebral tissue were detected with in situ hybridization （ISH） and immunohistochemistry （IHC）. The content of MDA and activity of Na^＋, K^＋-ATPase were detected. Results The space around the neurons and perivascular space became larger. Some neurons showed necrosis phenotype, and the number of the neurogliocyte increased. The expression of AQP4 and its mRNA were up-regulated in subfomical organ （SFO）, choroid plexes, brain cortex, supraoptic nuclei, hippocampus, and other regions, and reached its peak at 48-72 h in the model group after MCAO. The BWC in ischemic cerebral tissue reached its peak at 72 h. After operation, the morphological phenotype was not significantly changed in the Sham group and the salidroside group. BWC, MDA level, content changes of AQP4, and its mRNA expression were significantly lower than those in the model group. However, the activity of Na^＋, K^＋-ATPase were siginificant higher than that in the model group. Conclusion Salidroside could effeciently suppress the over-expression of AQP4 and its mRNA in the ischemic cerebral tissues of rats which result in the formation of ischemic cerebral edema.
出处
《中草药》
CAS
CSCD
北大核心
2008年第8期1200-1204,共5页
Chinese Traditional and Herbal Drugs
基金
重庆市教委科技基金资助项目(KJ050307)
重庆医科大学启动基金资助项目(QD200314)
