摘要
目的:研究红景天(Rhodiola)对体外培养肝癌QGY-7703的直接抑制作用及可能机制。方法:通过液体培养法、克隆形成法、3H-TdR掺入法与MTT比色法分别检测给药后肿瘤细胞的增殖情况、克隆形成率、DNA合成抑制率及生存率来探讨红景天的抑瘤效应。结果:与红景天共育24h的细胞伸展不良,胞体回缩,贴附型细胞不贴壁,胞质粗糙,有大量颗粒状物堆积,而且药物浓度越大,形态学改变越明显。给药组肿瘤细胞增殖缓慢甚至停滞,出现细胞脱落、胞浆内颗粒状物堆积等形态学改变,克隆形成数明显少于对照组,cpm和A值明显降低即3H-TdR掺入率减少,生存率下降。结论:红景天对体外培养肝癌细胞均具有直接杀伤作用。
Objective to elucidate the directed inhibiting effect and its mechanism of P.hodiola on hepatic carcinoma QGY-7703 cell. Method We detect the multiplication, clone formation, DNA synthesis inhibitive rate and survival rate by liquid cultivation, clone formation, 3H-TdR. addition and MTT colormetric method, Results Phodiola tardy the cell multiplication, decrease the clone formation, lessen 3H-TdP. addition and decrease the survival rate. Conclusion Rhodiola have cellular poison effect on hepatic carcinoma QGY-7703 cell. The inhibition effect show concentrition-depend.
出处
《吉林医学》
CAS
2005年第12期1285-1286,共2页
Jilin Medical Journal
