摘要
目的探讨视黄醇结合蛋白4(RBP4)的血清水平及-G803ASNP与代谢综合征的关系。方法收集116名伴2型糖尿病的代谢综合征患者和93名正常体检者,放射免疫法测定RBP4血清水平,聚合酶链式反应及序列分析检测G 803 A多态性基因型。结果(1)RBP4水平在所有受试者中与体质指数(BMI)、腰围、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、三酰甘油(TG)正相关,对照组中与TG、TC、LDL-C正相关,男性组中与DBP正相关,女性组中与年龄、FINS、HOMA-IR、TG、SBP正相关,多元逐步回归分析发现腰围、BMI、TG和年龄是独立相关因素;RBP4水平在代谢综合征组和男性组显著升高,在有规律运动组显著降低。(2)代谢综合征组与正常对照组的RBP4-G803A多态性基因型分布未见显著差异,按不同基因型分组的代谢指标亦无显著性差异。结论在中国汉族人群中,RBP4水平与多个代谢参数相关,RBP4基因-G 803 ASNP与代谢综合征没有关联。
Objective To investigate the relation between retinol binding protein-4 （RBP4） levels and metabolic parameters and to explore the correlation between RBP4-G 803 A SNP and metabolic syndrome. Methods One hundred and sixteen metabolic syndrome （MS） patients with type 2 diabetes mellitus and 93 healthy controls were included. Serum RBP4 level was measured by RIA. RBP4-G 803 A different genotypes were examined by PCR and sequence analysis. Results RBP4 levels showed significant positive correlations with BMI, waist circumference, FINS, HOMA-IR, and TG in all the participants, with TG, TC, and LDL-C in control, with DBP in males, and with age, FINS, HOMA-IR, TG and SBP in females. In multiple stepwise regression analysis, RBP4 was inde- pendently associated with BMI, waist circumference, TG and age. Serum RBP4 concentrations were significantly higher in MS group than in control. RBP4 levels were higher in males as compared to females. In control, RBP4 level was lower in subjects with regular exercise. There was no significant difference in the frequency of RBP4-G 803 A genotypes and alleles in MS group and neither difference in metabolic parameters between the carriers of GG ＋ GA and AA. Conclusion In Chinese Han population, serum RBP4 concentrations are correlated with various metabolic parameters and there is no relation between RBP4-G 803 A SNP and metabolic syndrome.
出处
《基础医学与临床》
CSCD
北大核心
2008年第7期742-746,共5页
Basic and Clinical Medicine
